Anti-CDK1 Antibody (Monoclonal, PSTAIR)

Boster Bio Anti-CDK1 Antibody (Monoclonal, PSTAIR) catalog # MA1087. Tested in WB applications. This antibody reacts with Human, Mouse. Cited in 9 publication(s).

Product Info Summary

SKU: MA1087
Size: 100μg/vial
Reactive Species: Human, Mouse
Host: Mouse
Application: WB

Product Name

Anti-CDK1 Antibody (Monoclonal, PSTAIR)

See all CDC2/CDK1 products

SKU/Catalog Number

MA1087

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CDK1 Antibody (Monoclonal, PSTAIR) catalog # MA1087. Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CDK1 Antibody (Monoclonal, PSTAIR) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1087)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

PSTAIR

Isotype

Mouse IgG1

Immunogen

Synthetic 16 amino acid oligopeptide containing the CDK1 sequence conjugated to BSA.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1087 is reactive to Cdk1 in Human, Mouse

Applications

MA1087 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, mouse

Validation Images & Assay Conditions

Gene/Protein Information For Cdk1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P39951

Gene ID

54237

Gene Name

Cdk1

Full Name

Cyclin-dependent kinase 1

Weight

34.135kDa

Superfamily

protein kinase superfamily

Alternative Names

CDC2; CDC28A; CDC2MGC111195; CDK1; cell cycle controller CDC2; Cell division control protein 2 homolog; Cell division protein kinase 1; cyclin-dependent kinase 1; DKFZp686L20222; EC 2.7.11.22; EC 2.7.11.23; G1 to S and G2 to M; p34 protein kinase; P34CDC2 Cdk1|Cdc2, Cdc2a|cyclin-dependent kinase 1|cyclin-dependent kinase 1|Cell division cycle control protein 2|cell division control protein 2 homolog|cell division cycle 2 homolog A|cell division cycle 2, G1 to S and G2 to M|cell division protein kinase 1|p34 protein kinase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cdk1, check out the Cdk1 Infographic

Cdk1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cdk1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1087 has been cited in 9 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

A novel cell cycle blocker extracted from Stellera chamaejasme L. inhibits the proliferation of hepatocarcinoma cells

Human enterovirus 68 interferes with the host cell cycle to facilitate viral production

Coxsackievirus A6 induces cell cycle arrest in G0/G1 phase for viral production

Yang C, Yang J, Sun M, Yan J, Meng X, Ma T. Iubmb Life. 2013 May;65(5):435-44. Doi: 10.1002/Iub.1141. Epub 2013 Feb 26. Alantolactone Inhibits Growth Of K562/Adriamycin Cells By Downregulating Bcr/Abl And P-Glycoprotein Expression.

Zeng Zj, Xiang Sg, Xue Ww, Li Hd, Ma N, Ren Zj, Xu Zj, Jiao Ch, Wang Cy, Hu Wx. Biomed Pharmacother. 2014 Sep;68(7):887-92. Doi: 10.1016/J.Biopha.2014.07.018. Epub 2014 Jul 28. The Cell Death And Dna Damages Caused By The Tet-On Regulating Hsv-Tk/...

Ye Z, Chen Z, Chen W, Xie J, Yang H, Lou Y, Yu Y. Chem Biol Interact. 2010 Jan 5;183(1):133-41. Doi: 10.1016/J.Cbi.2009.10.015. Xjw20, A Novel Oxoindole Derivative, Induces G2/M Arrest And Apoptosis Selectively In K562 Leukemia Cell Line.

Zheng Q, Liu Y, Liu W, Ma F, Zhou Y, Chen M, Chang J, Wang Y, Yang G, He G. Mol Med Rep. 2014 Jul;10(1):89-94. Doi: 10.3892/Mmr.2014.2175. Epub 2014 Apr 24. Cucurbitacin B Inhibits Growth And Induces Apoptosis Through The Jak2/Stat3 And Mapk Pathw...

Lin P, Fu J, Zhao B, Lin F, Zou H, Liu L, Zhu C, Wang H, Yu X. Mol Biol Rep. 2011 Mar;38(3):1741-7. Doi: 10.1007/S11033-010-0288-7. Epub 2010 Sep 29. Fbxw8 Is Involved In The Proliferation Of Human Choriocarcinoma Jeg-3 Cells.

Ren J, Huang Q, Xu Y, Yang M, Yang J, Hu K. Anticancer Drugs. 2015 Jul;26(6):599-611. Doi: 10.1097/Cad.0000000000000224. Isoflavone Lupiwighteone Induces Cytotoxic, Apoptotic, And Antiangiogenic Activities In Du-145 Prostate Cancer Cells.

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15 Customer Q&As for Anti-CDK1 Antibody (Monoclonal, PSTAIR)

Question

Will MA1087 anti-CDK1 antibody (Monoclonal, PSTAIR) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-12

Answer

As indicated on the product datasheet, MA1087 anti-CDK1 antibody (Monoclonal, PSTAIR) as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-12

Question

Will anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087 work on pig WB with leukemic t-cell?

Verified Customer

Verified customer

Asked: 2019-12-25

Answer

Our lab technicians have not validated anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087 on pig. You can run a BLAST between pig and the immunogen sequence of anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig leukemic t-cell in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-25

Question

Would anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087 work for WB with intestine?

Verified Customer

Verified customer

Asked: 2019-12-20

Answer

According to the expression profile of intestine, CDK1 is highly expressed in intestine. So, it is likely that anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087 will work for WB with intestine.

Boster Scientific Support

Answered: 2019-12-20

Question

We want to test anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087 on mouse intestine for research purposes, then I may be interested in using anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-11-01

Answer

The products we sell, including anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-11-01

Question

We are currently using anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-30

Answer

The anti-CDK1 antibody (Monoclonal, PSTAIR) (MA1087) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-30

Question

We have observed staining in mouse cervix carcinoma. Do you have any suggestions? Is anti-CDK1 antibody (Monoclonal, PSTAIR) supposed to stain cervix carcinoma positively?

Verified Customer

Verified customer

Asked: 2019-09-23

Answer

According to literature cervix carcinoma does express CDK1. According to Uniprot.org, CDK1 is expressed in intestine, mammary cancer, skin, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-09-23

Question

I was wanting to use your anti-CDK1 antibody (Monoclonal, PSTAIR) for WB for mouse intestine on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse intestine identification?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

You can see on the product datasheet, MA1087 anti-CDK1 antibody (Monoclonal, PSTAIR) has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse intestine in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-09

Question

Is there a BSA free version of anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087 available?

M. Williams

Verified customer

Asked: 2019-07-12

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-12

Question

We were well pleased with the WB result of your anti-CDK1 antibody (Monoclonal, PSTAIR). However we have seen positive staining in cervix carcinoma nucleus. cytoplasm. mitochondrion. using this antibody. Is that expected? Could you tell me where is CDK1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-12-07

Answer

From what I have seen in literature, cervix carcinoma does express CDK1. Generally CDK1 expresses in nucleus. cytoplasm. mitochondrion. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-12-07

Question

I see that the anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-04-24

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-04-24

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for intestine using anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-10-18

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-10-18

Question

Is a blocking peptide available for product anti-CDK1 antibody (Monoclonal, PSTAIR) (MA1087)?

Z. Miller

Verified customer

Asked: 2016-06-14

Answer

We do provide the blocking peptide for product anti-CDK1 antibody (Monoclonal, PSTAIR) (MA1087). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2016-06-14

Question

My question regarding product MA1087, anti-CDK1 antibody (Monoclonal, PSTAIR). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

L. Johnson

Verified customer

Asked: 2015-03-19

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1087 anti-CDK1 antibody (Monoclonal, PSTAIR), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2015-03-19

Question

Is this MA1087 anti-CDK1 antibody (Monoclonal, PSTAIR) reactive to the isotypes of CDK1?

J. Banerjee

Verified customer

Asked: 2014-02-21

Answer

The immunogen of MA1087 anti-CDK1 antibody (Monoclonal, PSTAIR) is Synthetic 16 amino acid oligopeptide containing the CDK1 sequence conjugated to BSA. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-02-21

Question

Here is the WB image, lot number and protocol we used for intestine using anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087. Please let me know if you require anything else.

M. Walker

Verified customer

Asked: 2013-10-15

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-10-15

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