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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-CDK1 Antibody (Monoclonal, PSTAIR)
Boster Bio Anti-CDK1 Antibody (Monoclonal, PSTAIR) catalog # MA1087. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDK1 Antibody (Monoclonal, PSTAIR) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1087)
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Synthetic 16 amino acid oligopeptide containing the CDK1 sequence conjugated to BSA.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross-reactivity with other proteins
MA1087 is reactive to Cdk1 in Human, Mouse, Rat
MA1087 is guaranteed for WB Boster Guarantee
Background of CDC2/CDK1
The cyclin-dependent protein kinases (CDKs) regulate major cell cycle transitions in eukaryotic cells. CDKs contain an evolutionary conserved 16 amino acid sequence called PSTAIR (EGVPSTAIREISLLKE) which distinguishes them from other protein kinases. The PSTAIRE motif found in prototypic CDC2 kinases. CDC2L1 is referred as PITSLRE B, based on the amino acid sequence of the region corresponding to the conserved CDC2 PSTAIRE box.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.5-1μg/ml, Human, mouse, rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CDK1 using anti-CDK1 antibody (MA1087).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human CACO-2 whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse RAW264.7 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CDK1 antigen affinity purified monoclonal antibody (Catalog # MA1087) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CDK1 at approximately 34 kDa. The expected band size for CDK1 is at 34 kDa.
Gene/Protein Information For Cdk1 (Source: Uniprot.org, NCBI)
Cyclin-dependent kinase 1
protein kinase superfamily
CDC2; CDC28A; CDC2MGC111195; CDK1; cell cycle controller CDC2; Cell division control protein 2 homolog; Cell division protein kinase 1; cyclin-dependent kinase 1; DKFZp686L20222; EC 188.8.131.52; EC 184.108.40.206; G1 to S and G2 to M; p34 protein kinase; P34CDC2 Cdk1|Cdc2, Cdc2a|cyclin-dependent kinase 1|cyclin-dependent kinase 1|Cell division cycle control protein 2|cell division control protein 2 homolog|cell division cycle 2 homolog A|cell division cycle 2, G1 to S and G2 to M|cell division protein kinase 1|p34 protein kinase*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on Cdk1, check out the Cdk1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cdk1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
MA1087 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A novel cell cycle blocker extracted from Stellera chamaejasme L. inhibits the proliferation of hepatocarcinoma cells
Human enterovirus 68 interferes with the host cell cycle to facilitate viral production
Coxsackievirus A6 induces cell cycle arrest in G0/G1 phase for viral production
Yang C, Yang J, Sun M, Yan J, Meng X, Ma T. Iubmb Life. 2013 May;65(5):435-44. Doi: 10.1002/Iub.1141. Epub 2013 Feb 26. Alantolactone Inhibits Growth Of K562/Adriamycin Cells By Downregulating Bcr/Abl And P-Glycoprotein Expression.
Zeng Zj, Xiang Sg, Xue Ww, Li Hd, Ma N, Ren Zj, Xu Zj, Jiao Ch, Wang Cy, Hu Wx. Biomed Pharmacother. 2014 Sep;68(7):887-92. Doi: 10.1016/J.Biopha.2014.07.018. Epub 2014 Jul 28. The Cell Death And Dna Damages Caused By The Tet-On Regulating Hsv-Tk/...
Ye Z, Chen Z, Chen W, Xie J, Yang H, Lou Y, Yu Y. Chem Biol Interact. 2010 Jan 5;183(1):133-41. Doi: 10.1016/J.Cbi.2009.10.015. Xjw20, A Novel Oxoindole Derivative, Induces G2/M Arrest And Apoptosis Selectively In K562 Leukemia Cell Line.
Zheng Q, Liu Y, Liu W, Ma F, Zhou Y, Chen M, Chang J, Wang Y, Yang G, He G. Mol Med Rep. 2014 Jul;10(1):89-94. Doi: 10.3892/Mmr.2014.2175. Epub 2014 Apr 24. Cucurbitacin B Inhibits Growth And Induces Apoptosis Through The Jak2/Stat3 And Mapk Pathw...
Lin P, Fu J, Zhao B, Lin F, Zou H, Liu L, Zhu C, Wang H, Yu X. Mol Biol Rep. 2011 Mar;38(3):1741-7. Doi: 10.1007/S11033-010-0288-7. Epub 2010 Sep 29. Fbxw8 Is Involved In The Proliferation Of Human Choriocarcinoma Jeg-3 Cells.
Ren J, Huang Q, Xu Y, Yang M, Yang J, Hu K. Anticancer Drugs. 2015 Jul;26(6):599-611. Doi: 10.1097/Cad.0000000000000224. Isoflavone Lupiwighteone Induces Cytotoxic, Apoptotic, And Antiangiogenic Activities In Du-145 Prostate Cancer Cells.
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