Anti-Cyclin-dependent kinase 9 Cdk9 Antibody

Product Name

Anti-Cyclin-dependent kinase 9 Cdk9 Antibody

View all CDK9 Antibodies

SKU/Catalog Number

A00794

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Cyclin-dependent kinase 9 Cdk9 Antibody (Catalog # A00794). Tested in ELISA, IHC, IP, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Cyclin-dependent kinase 9 Cdk9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00794)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

Multiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the human gene cdk9 (PITALRE).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00794 is reactive to CDK9 in Human, Mouse

Applications

A00794 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

42.778kDa

Background of CDK9

CDK9 (PITALRE) (also known as cyclin-dependent kinase 9, Serine/threonine-protein kinase PITALRE, C-2K and Cell division cycle 2-like protein kinase 4) is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. CDK9 (PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2. This kinase also was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. Tat stimulates human HIV-1 viral transcription elongation. This suggests that cyclin T1/cdk9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation. Cyclin T1/cdk9(PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb. The cdk9(PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev. CDK9 (PITALRE) is a ubiquitously expressed nuclear protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
IHC: 1:200 - 1:1,000
IP: 1:100
WB: 1:500 - 1:3,000

Validation Images & Assay Conditions

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Cdk9 was detected in paraffin-embedded sections of mouse tissue using rabbit anti-Cdk9 antiserum polyclonal antibody (Catalog # A00794) at 1:500. Panel A: Peroxidase-DAB immunostaining of mcdk9/PITALRE protein in the developing mouse brain in the differentiated region of the medulla oblongata just below the fourth ventricle. Similar staining is shown in Panel B in the dorsal root ganglia. Panel C: Fluorescein immunofluorescence of mcdk9IPITALRE in skeletal muscle. Similar staining is shown in Panel D in cardiac muscle. The immunohistochemical section was developed using SABC method (Catalog # SA1022).

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Western blot analysis of Cdk9 expression in PC3 whole cell lysates (lane 1) and DU145 prostate cancer cell lysates (lane 2). Cdk9 at 55 and 42KD was detected using rabbit anti-Cdk9 antiserum polyclonal antibody (Catalog # A00794) at 1:1500. Personal communication Flavio Rizzolio, Temple University

Gene/Protein Information For CDK9 (Source: Uniprot.org, NCBI)

Alternative Names

C-2k; CDC2L4CTK1; CDC2-related kinase; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; cyclin-dependent kinase 9 (CDC2-related kinase); cyclin-dependent kinase 9; EC 2.7.11; EC 2.7.11.22; EC 2.7.11.23; PITALRE; serine/threonine protein kinase PITALRE; Serine/threonine-protein kinase PITALRE; TAK CDK9 C-2k, CDC2L4, CTK1, PITALRE, TAK cyclin dependent kinase 9 cyclin-dependent kinase 9|CDC2-related kinase|cell division cycle 2-like protein kinase 4|cell division protein kinase 9|serine/threonine protein kinase PITALRE|tat-associated kinase complex catalytic subunit

For more info on CDK9, check out the CDK9 Infographic

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In this infographic, you will see the following information for CDK9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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