Anti-CELA3B / ELA3B (Pancreatic Function Marker) Monoclonal Antibody

Product Name

Anti-CELA3B / ELA3B (Pancreatic Function Marker) Monoclonal Antibody

View all Elastase Antibodies

SKU/Catalog Number

M09940

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-CELA3B / ELA3B (Pancreatic Function Marker) Monoclonal Antibody (Catalog # M09940). Tested in ELISA, WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-CELA3B / ELA3B (Pancreatic Function Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09940)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: CELA3B/1257

Isotype

IgG1, kappa

Immunogen

Recombinant human CELA3B protein fragment (around aa 82-238) (exact sequence is proprietary)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M09940 is reactive to CELA3B in Human, Mouse, Rat

Applications

M09940 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

29.263kDa

Background of Elastase

This monoclonal antibody recognizes a protein of ~30kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Pancreas stained with Anti-CELA3B Mouse Monoclonal Antibody (CELA3B/1257)

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Formalin-fixed, paraffin-embedded Rat Pancreas stained with Anti-CELA3B Mouse Monoclonal Antibody (CELA3B/1257).

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Formalin-fixed, paraffin-embedded Mouse Pancreas stained with Anti-CELA3B Mouse Monoclonal Antibody (CELA3B/1257).

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Western Blot analysis of PANC-1 cell lysate using Anti-CELA3B Mouse Monoclonal Antibody (CELA3B/1257).

Gene/Protein Information For CELA3B (Source: Uniprot.org, NCBI)

Alternative Names

CBPP; cholesterol-binding pancreatic protease; chymotrypsin-like elastase family member 3B; chymotrypsin-like elastase family, member 3B; E1; EC 3.4.21; EC 3.4.21.70; EL-1; ELA3B; elastase 1; elastase 3B, pancreatic; Elastase IIIB; elastase-3B; fecal elastase 1; pancreatic elastase 1; pancreatic endopeptidase E; Protease E; proteinase E CELA3B CBPP, ELA3B chymotrypsin like elastase 3B chymotrypsin-like elastase family member 3B|cholesterol-binding pancreatic protease|chymotrypsin like elastase family member 3B|elastase 3B, pancreatic|elastase IIIB|elastase-3B|fecal elastase 1|pancreatic elastase 1|pancreatic endopeptidase E|protease E|proteinase E

For more info on CELA3B, check out the CELA3B Infographic

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In this infographic, you will see the following information for CELA3B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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