Anti-CEP290 (F812) Antibody

Product Name

Anti-CEP290 (F812) Antibody

View all CEP290 Antibodies

SKU/Catalog Number

A01894

Size

100ul

Form

Liquid

Description

Boster Bio Anti-CEP290 (F812) Antibody catalog # A01894. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CEP290 (F812) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01894)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human MDMX around the phosphorylation site of S367.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01894 is reactive to CEP290 in Human, Mouse, Rat

Applications

A01894 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

290.386kDa

Background of CEP290

Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.

McKay M.J., Genomics 36:305-315(1996).
Sadano H., Biochem. Biophys. Res. Commun. 267:418-422(2000).
Nomura N., DNA Res. 1:223-229(1994).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-CEP290 (F812) Antibody A01894

Western blot (WB) analysis of CEP290 (F812) polyclonal antibody at 1:500 dilution
Lane1:HCT116 whole cell lysate(40ug)
Lane2:SGC7901 whole cell lysate(40ug)
Lane3:COS-7 whole cell lysate(40ug)
Lane4:A549 whole cell lysate(40ug)
Lane5:CT26 whole cell lysate(40ug)
Lane6:AML-12 whole cell lysate(40ug)
Lane7:PC12 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For CEP290 (Source: Uniprot.org, NCBI)

Alternative Names

BBS14Bardet-Biedl syndrome 14 protein; Cancer/testis antigen 87; centrosomal protein 290kDa; Cep290; CT87JBTS6; FLJ13615; JBTS5CTCL tumor antigen se2-2; KIAA0373FLJ21979; LCA10monoclonal 3H11 antigen; MKS4; Nephrocystin-6; NPHP6centrosomal protein of 290 kDa; POC3 centriolar protein homolog; POC3; prostate cancer antigen T21; rd16,3H11AG; SLSN6nephrocytsin-6,3H11Ag; Tumor antigen se2-2 CEP290 3H11Ag, BBS14, CT87, JBTS5, LCA10, MKS4, NPHP6, POC3, SLSN6, rd16 centrosomal protein 290 centrosomal protein of 290 kDa|Bardet-Biedl syndrome 14 protein|CTCL tumor antigen se2-2|Meckel syndrome, type 4|POC3 centriolar protein homolog|cancer/testis antigen 87|centrosomal protein 290kDa|monoclonal antibody 3H11 antigen|nephrocytsin-6|prostate cancer antigen T21|tumor antigen se2-2

For more info on CEP290, check out the CEP290 Infographic

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In this infographic, you will see the following information for CEP290: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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