Anti-CFA/TBCA Antibody Picoband™

Product Name

Anti-CFA/TBCA Antibody Picoband™

View all TBCA Antibodies

SKU/Catalog Number

A06926-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CFA/TBCA Antibody Picoband™ catalog # A06926-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-CFA/TBCA Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06926-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CFA/TBCA recombinant protein (Position: M1-A108).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A06926-1 is reactive to TBCA in Human

Applications

A06926-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

18 kDa

Calculated molecular weight

12.855kDa

Background of TBCA

Tubulin-specific chaperone A is a protein that in humans is encoded by the TBCA gene. The product of this gene is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. This gene encodes chaperonin cofactor A. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of CFA/TBCA using anti-CFA/TBCA antibody (A06926-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CFA/TBCA antigen affinity purified polyclonal antibody (Catalog # A06926-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CFA/TBCA at approximately 18 kDa. The expected band size for CFA/TBCA is at 18 kDa.

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Figure 2. Flow Cytometry analysis of THP-1 cells using anti-CFA/TBCA antibody (A06926-1).
Overlay histogram showing THP-1 cells stained with A06926-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CFA/TBCA Antibody (A06926-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For TBCA (Source: Uniprot.org, NCBI)

Alternative Names

CFA; chaperonin cofactor A; TCP1-chaperonin cofactor A; tubulin folding cofactor A; Tubulin-folding cofactor A; tubulin-specific chaperone a TBCA tubulin folding cofactor A tubulin-specific chaperone A|CFA|TCP1-chaperonin cofactor A|chaperonin cofactor A|epididymis secretory sperm binding protein

For more info on TBCA, check out the TBCA Infographic

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In this infographic, you will see the following information for TBCA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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