Anti-Clathrin Heavy Chain Purified CLTC Monoclonal Antibody

Product Name

Anti-Clathrin Heavy Chain Purified CLTC Monoclonal Antibody

View all Clathrin Heavy Chain 1/CHC17 Antibodies

SKU/Catalog Number

M03134-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Clathrin Heavy Chain Purified CLTC Monoclonal Antibody (Catalog# M03134-1). Tested in Flow Cytometry, IP, WB, ICC, ELISA application(s). This antibody reacts with Rat, Cow, Mouse, Human, Pig.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Clathrin Heavy Chain Purified CLTC Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03134-1)

Host

Mouse

Contents

Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

BF-06

Isotype

Mouse IgM

Immunogen

The antibody BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa intracellular protein involved in receptor-mediated endocytosis.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M03134-1 is reactive to CLTC in Cow, Human, Mouse, Pig, Rat

Applications

M03134-1 is guaranteed for ELISA, Flow Cytometry, IP, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

191.615kDa

Background of Clathrin Heavy Chain 1/CHC17

Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 2-5 μg/ml; positive control: human blood leukocytes. Intracellular staining.

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Immunocytochemistry staining of CLTC using Anti-Clathrin Heavy Chain Purified CLTC Monoclonal Antibody (M03134-1).

Immunocytochemistry staining of clathrin in human primary fibroblasts using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Click image to see more details

Figure 2. Immunocytochemistry staining of CLTC using Anti-Clathrin Heavy Chain Purified CLTC Monoclonal Antibody (M03134-1).

Immunocytochemistry staining of clathrin in human HeLa cell line using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Gene/Protein Information For CLTC (Source: Uniprot.org, NCBI)

Alternative Names

CHC; CHC17; Clathrin Heavy Chain 1; Clathrin heavy chain on chromosome 17; clathrin, heavy chain (Hc); clathrin, heavy chain; clathrin, heavy polypeptide (Hc); clathrin, heavy polypeptide-like 2; CLH17; CLH-17; CLTC; CLTCL2; Hc; KIAA0034 CLTC CHC, CHC17, CLH-17L2, Hc, MRD56, CLTC clathrin heavy chain clathrin heavy chain 1|clathrin heavy chain on chromosome 17|clathrin, heavy polypeptide (Hc)|clathrin, heavy polypeptide-like 2

For more info on CLTC, check out the CLTC Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CLTC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the CLTC Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis