Product Info Summary
SKU: | M03683 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Claudin 4 Monoclonal Antibody
SKU/Catalog Number
M03683
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Claudin 4 Monoclonal Antibody catalog # M03683. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Claudin 4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03683)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADEB-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Claudin 4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03683 is reactive to CLDN4 in Human
Applications
M03683 is guaranteed for IP, IHC, WB Boster Guarantee
Observed Molecular Weight
120 kDa
Calculated molecular weight
22.077kDa
Background of Claudin-4
Plays a major role in tight junction-specific obliteration of the intercellular space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:100-1:500
IP 1:20
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Claudin 4 expression in MCF7 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CLDN4 (Source: Uniprot.org, NCBI)
Gene Name
CLDN4
Full Name
Claudin-4
Weight
22.077kDa
Superfamily
claudin family
Alternative Names
claudin 4; Claudin4; Claudin-4; CLDN4; Clostridium perfringens enterotoxin receptor 1; Clostridium perfringens enterotoxin receptor; CPE-R; CPE-RCPETR; CPE-receptor; CPETR1; hCPE-R; WBSCR8; WBSCR8CPERCPETR1claudin-4; Williams-Beuren syndrome chromosomal region 8 protein CLDN4 CPE-R, CPER, CPETR, CPETR1, WBSCR8, hCPE-R claudin 4 claudin-4|CPE-receptor|Clostridium perfringens enterotoxin receptor 1|Williams-Beuren syndrome chromosomal region 8 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CLDN4, check out the CLDN4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CLDN4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Claudin 4 Monoclonal Antibody (M03683)
Hello CJ!
No publications found for M03683
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Claudin 4 Monoclonal Antibody
Question
I have a question about product M03683, anti-Claudin 4 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03683 anti-Claudin 4 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-27
Question
Does M03683 anti-Claudin 4 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-09-28
Answer
It shows on the product datasheet, M03683 anti-Claudin 4 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-09-28
Question
We are currently using anti-Claudin 4 Monoclonal antibody M03683 for human tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
N. Edwards
Verified customer
Asked: 2016-01-01
Answer
The anti-Claudin 4 Monoclonal antibody (M03683) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-01-01