Anti-CNPase Antibody Picoband™

Product Name

Anti-CNPase Antibody Picoband™

View all CNPase Antibodies

SKU/Catalog Number

A01017

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CNPase Antibody Picoband™ catalog # A01017. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CNPase Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01017)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human CNPase, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01017 is reactive to CNP in Human, Mouse, Rat

Applications

A01017 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

47 kDa

Calculated molecular weight

47.579kDa

Background of CNPase

2',3'-Cyclic-nucleotide 3'-phosphodiesterase, also known as CNPase, is an enzyme that in humans is encoded by the CNP gene. And this gene is mapped to 17q21.2. CNPase is named for its ability to catalyze the phosphodiester hydrolysis of 2',3'-cyclic nucleotides to 2'-nucleotides. CNPase is thought to play a critical role in the events leading up to myelination. Additionally, CNPase has been demonstrated to inhibit the replication of HIV-1 and other primate lentiviruses by binding the retroviral Gag protein and inhibiting the genesis of nascent viral particles.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of CNPase using anti-CNPase antibody (A01017).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: rat brain tissue lysates,
lane 2: mouse brain tissue lysates,
lane 3: HELA whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CNPase antigen affinity purified polyclonal antibody (Catalog # A01017) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CNPase at approximately 47KD. The expected band size for CNPase is at 47KD.

Gene/Protein Information For CNP (Source: Uniprot.org, NCBI)

Alternative Names

2', 3' cyclic nucleotide 3' phosphohydrolase; 2'-3'-cyclic nucleotide 3' phosphodiesterase; 2'-3'-cyclic-nucleotide 3'-phosphodiesterase; CNP1; CNPase; EC 3.1.4.37 CNP CNP1, HLD20 2,3-cyclic nucleotide 3 phosphodiesterase 2,3-cyclic-nucleotide 3-phosphodiesterase|2, 3 cyclic nucleotide 3 phosphohydrolase|CNPase

For more info on CNP, check out the CNP Infographic

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In this infographic, you will see the following information for CNP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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