Anti-COL6A1 Antibody (C-term)

Product Name

Anti-COL6A1 Antibody (C-term)

View all Collagen VI alpha 1 Antibodies

SKU/Catalog Number

A02226-4

Size

80 µl

Description

Boster Bio Anti-COL6A1 Antibody (C-term) (Catalog # A02226-4). Tested in WB, IF, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-COL6A1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02226-4)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This COL6A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 831-858 amino acids from the C-terminal region of human COL6A1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02226-4 is reactive to COL6A1 in Human

Applications

A02226-4 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

108.529kDa

Background of Collagen VI alpha 1

The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The protein COL6A1 is the alpha 1 subunit of type VI collagen (alpha1(VI) chain). Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:10-1:50
WB: 1:500-1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50

Validation Images & Assay Conditions

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Confocal immunofluorescent analysis of COL6A1 Antibody (C-term) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

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Western blot analysis of COL6A1 antibody (C-term) in Y79 cell line lysates (35ug/lane). COL6A1 (arrow) was detected using the purified Pab.

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Western blot analysis of lysate from A431 cell line, using COL6A1 Antibody (C-term). A02226-4 diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

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Formalin-fixed and paraffin-embedded human hepatocarcinoma with COL6A1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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Flow cytometric analysis of 293 cells using COL6A1 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Gene/Protein Information For COL6A1 (Source: Uniprot.org, NCBI)

Alternative Names

Collagen 6; collagen alpha-1(VI) chain; collagen VI, alpha-1 polypeptide; collagen, type VI, alpha 1; Collagen-6; human mRNA for collagen VI alpha-1 C-terminal globular domain10alpha 1 (VI) chain (61 AA); OPLL COL6A1 BTHLM1, OPLL, UCHMD1 collagen type VI alpha 1 chain collagen alpha-1(VI) chain|alpha 1 (VI) chain (61 AA)|collagen VI, alpha-1 polypeptide|collagen, type VI, alpha 1|epididymis secretory sperm binding protein

For more info on COL6A1, check out the COL6A1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for COL6A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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