Anti-Cyclin A1/A2 Rabbit Monoclonal Antibody

Product Name

Anti-Cyclin A1/A2 Rabbit Monoclonal Antibody

View all Cyclin A2 Antibodies

SKU/Catalog Number

M00700

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Cyclin A1/A2 Rabbit Monoclonal Antibody catalog # M00700. Tested in WB, IHC, IP applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cyclin A1/A2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00700)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

HOD-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Cyclin A1/A2

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00700 is reactive to CCNA2 in Human

Applications

M00700 is guaranteed for IP, IHC, WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

48.551kDa

Background of Cyclin A2

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:1000
IHC 1:100-1:500
IP 1:50

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Cyclin A1/A2 using anti-Cyclin A1/A2 antibody (M00700).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin A1/A2 antigen affinity purified polyclonal antibody (Catalog # M00700) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin A1/A2 at approximately 55 kDa. The expected band size for Cyclin A1/A2 is at 49 kDa.

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Immunohistochemical analysis of paraffin-embedded human colon cancer, using Cyclin A1/A2 Antibody.

Gene/Protein Information For CCNA2 (Source: Uniprot.org, NCBI)

Alternative Names

CCN1CCNAcyclin-A; CCNA; CCNA2; Cyclin A2; Cyclin-A; cyclin-A2 CCNA2 CCN1, CCNA cyclin A2 cyclin-A2|cyclin-A

For more info on CCNA2, check out the CCNA2 Infographic

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In this infographic, you will see the following information for CCNA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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