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Product Info Summary
| SKU: | M00700 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Cyclin A1/A2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00700
BM4674 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cyclin A1/A2 Rabbit Monoclonal Antibody catalog # M00700. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin A1/A2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00700)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HOD-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cyclin A1/A2
Reactive Species
M00700 is reactive to CCNA2 in Human
Observed Molecular Weight
55 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00700 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
Positive Control
WB: human Hela whole cell, human Jurkat whole cell
IHC: human colon cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Cyclin A1/A2 using anti-Cyclin A1/A2 antibody (M00700).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin A1/A2 antigen affinity purified monoclonal antibody (Catalog # M00700) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin A1/A2 at approximately 55 kDa. The expected band size for Cyclin A1/A2 is at 49 kDa.
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Immunohistochemical analysis of paraffin-embedded human colon cancer, using Cyclin A1/A2 Antibody.
Specific Publications For Anti-Cyclin A1/A2 Rabbit Monoclonal Antibody (M00700)
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