Anti-Cyclin D1 CCND1 Rabbit Monoclonal Antibody

Product Name

Anti-Cyclin D1 CCND1 Rabbit Monoclonal Antibody

View all CCND1 Antibodies

SKU/Catalog Number

M00149-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Cyclin D1 CCND1 Rabbit Monoclonal Antibody catalog # M00149-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cyclin D1 CCND1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00149-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

DAD-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Cyclin D1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00149-1 is reactive to CCND1 in Human, Mouse, Rat

Applications

M00149-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

34 kDa

Calculated molecular weight

33.729kDa

Background of CCND1

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:100
IP 1:50

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Cyclin D1 using anti-Cyclin D1 antibody (M00149-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human LNCAP whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin D1 antigen affinity purified monoclonal antibody (Catalog # M00149-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin D1 at approximately 34 kDa. The expected band size for Cyclin D1 is at 34 kDa.

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Immunohistochemical analysis of paraffin-embedded human bladder, using Cyclin D1 Antibody.

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Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:100 dilution.

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Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma , using the Antibody at 1:100 dilution.

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Immunohistochemical analysis of paraffin-embedded Human ovarian cancer, using the Antibody at 1:500 dilution.

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Immunofluorescent analysis of MCF-7 cells, using Cyclin D1 Antibody.

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

Gene/Protein Information For CCND1 (Source: Uniprot.org, NCBI)

Alternative Names

B-cell lymphoma 1 protein; BCL-1 oncogene; Bcl-1; BCL1D11S287E; CCND1; cyclin D1 (PRAD1: parathyroid adenomatosis 1); Cyclin D1; Cyl-1; G1/S-specific cyclin D1; G1/S-specific cyclin-D1; PRAD1 oncogene; PRAD1; PRAD1B-cell CLL/lymphoma 1; U21B31 CCND1 BCL1, D11S287E, PRAD1, U21B31 cyclin D1 G1/S-specific cyclin-D1|B-cell CLL/lymphoma 1|B-cell lymphoma 1 protein|BCL-1 oncogene|PRAD1 oncogene

For more info on CCND1, check out the CCND1 Infographic

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In this infographic, you will see the following information for CCND1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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