Anti-DAP Kinase 1 DAPK1 Rabbit Monoclonal Antibody

DAP Kinase 1 antibody

Boster Bio Anti-DAP Kinase 1 DAPK1 Rabbit Monoclonal Antibody catalog # M01161. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: M01161
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, ICC, WB

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Product Name

Anti-DAP Kinase 1 DAPK1 Rabbit Monoclonal Antibody

View all DAP Kinase 1 Antibodies

SKU/Catalog Number

M01161

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-DAP Kinase 1 DAPK1 Rabbit Monoclonal Antibody catalog # M01161. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DAP Kinase 1 DAPK1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01161)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

COA-4

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human DAP Kinase 1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M01161 is reactive to DAPK1 in Human, Mouse, Rat

Applications

M01161 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

40 kDa

Calculated molecular weight

160.046kDa

Background of DAP Kinase 1

Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For DAPK1 (Source: Uniprot.org, NCBI)

Gene Name

DAPK1

Full Name

Death-associated protein kinase 1

Weight

160.046kDa

Superfamily

protein kinase superfamily

Alternative Names

DAPKDAP kinase 1; death-associated protein kinase 1; DKFZp781I035; EC 2.7.11; EC 2.7.11.1 DAPK1 DAPK, ROCO3 death associated protein kinase 1 death-associated protein kinase 1|DAP kinase 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DAPK1, check out the DAPK1 Infographic

DAPK1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DAPK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M01161 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Reduced expression levels of the death-associated protein kinase and E-cadherin are correlated with the development of esophageal squamous cell carcinoma

DRP-1, ezrin and E-cadherin expression and the association with esophageal squamous cell carcinoma

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6 Customer Q&As for Anti-DAP Kinase 1 DAPK1 Rabbit Monoclonal Antibody

Question

I see that the anti-DAP Kinase 1 Rabbit Monoclonal antibody M01161 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-25

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-25

Question

My question regarding product M01161, anti-DAP Kinase 1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-09-19

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01161 anti-DAP Kinase 1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-09-19

Question

Here is the WB image, lot number and protocol we used for amygdala using anti-DAP Kinase 1 Rabbit Monoclonal antibody M01161. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-16

Question

Would M01161 anti-DAP Kinase 1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-04-12

Answer

It shows on the product datasheet, M01161 anti-DAP Kinase 1 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-04-12

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amygdala using anti-DAP Kinase 1 Rabbit Monoclonal antibody M01161. Let me know if you need anything else.

K. Rodriguez

Verified customer

Asked: 2015-09-04

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-09-04

Question

We are currently using anti-DAP Kinase 1 Rabbit Monoclonal antibody M01161 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

B. Lewis

Verified customer

Asked: 2013-02-25

Answer

The anti-DAP Kinase 1 Rabbit Monoclonal antibody (M01161) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-02-25

Order DetailsPrice
M01161

100μL

$370
M01161-30ul

30μL sample

$99
M01161-carrier-free

Carrier Free

$370

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