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Product Info Summary
| SKU: | A02241 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-DAPK2 Antibody
View all DAP Kinase 2 Antibodies
SKU/Catalog Number
A02241
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-DAPK2 Antibody (Catalog # A02241). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
DAPK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-DAPK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02241)
Host
Rabbit
Contents
DAPK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DAPK2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DAPK2. This antigenic peptide is identical to the corresponding amino acids of mouse origin (1,2). The immunogen is located within the last 50 amino acids of DAPK2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
DAPK2 has no cross responses to DAPK1.
Reactive Species
A02241 is reactive to Dapk2 in Human, Mouse, Rat
Applications
A02241 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
42.778kDa
Background of DAP Kinase 2
Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFNγ induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1. Ectopicly expressed DAPK2 induced apoptosis in various types of cells. DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA of DAPK2 is expressed in multiple human tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DAPK2 antibody can be used for detection of DAPK2 by Western blot at 1 μg/mL. An approximately 42 kDa band can be detected. DAPK2 has no cross responses to DAPK1. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.
Antibody validated: Western Blot in human, mouse, and rat samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of DAPK2 in A431 (H)
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Immunohistochemistry of DAPK2 in mouse spleen cells with DAPK2 antibody at 2 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For Dapk2 (Source: Uniprot.org, NCBI)
Gene Name
Dapk2
Full Name
Death-associated protein kinase 2
Weight
42.778kDa
Superfamily
protein kinase superfamily
Alternative Names
DAP kinase 2; DAP-kinase-related protein 1 beta isoform; DAP-kinase-related protein 1; death-associated protein kinase 2; DRP1; DRP-1MGC119312; EC 2.7.11; EC 2.7.11.1 Dapk2|death-associated protein kinase 2|death-associated protein kinase 2|DAP kinase 2|DRP-1|death-associated kinase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Dapk2, check out the Dapk2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Dapk2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DAPK2 Antibody (A02241)
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