Anti-DAX-1/NR0B1 Antibody Picoband™

Product Name

Anti-DAX-1/NR0B1 Antibody Picoband™

View all DAX1/NR0B1 Antibodies

SKU/Catalog Number

A01521-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-DAX-1/NR0B1 Antibody Picoband™ catalog # A01521-2. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-DAX-1/NR0B1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01521-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human DAX-1/NR0B1 recombinant protein (Position: V126-Q404).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01521-2 is reactive to NR0B1 in Human

Applications

A01521-2 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

48 kDa

Calculated molecular weight

51.718kDa

Background of DAX1/NR0B1

DAX1 (dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1) is a nuclear receptor protein that in humans is encoded by the NR0B1 gene (nuclear receptor subfamily 0, group B, member 1). This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of DAX-1/NR0B1 using anti-DAX-1/NR0B1 antibody (A01521-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human U87 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAX-1/NR0B1 antigen affinity purified polyclonal antibody (Catalog # A01521-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAX-1/NR0B1 at approximately 48 kDa. The expected band size for DAX-1/NR0B1 is at 48 kDa.

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Figure 2. IF analysis of DAX-1/NR0B1 using anti-DAX-1/NR0B1 antibody (A01521-2) and anti-Beta Tubulin antibody (M01857-3).
DAX-1/NR0B1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DAX-1/NR0B1 Antibody (A01521-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For NR0B1 (Source: Uniprot.org, NCBI)

Alternative Names

AHC; AHCDSS; AHCH; AHX; DAX1; DAX1DAX-1; dosage-sensitive sex reversal; DSS-AHC critical region on the X chromosome protein 1; GTD; HHG; NR0B1; NROB1; nuclear hormone receptor; Nuclear receptor DAX-1; nuclear receptor subfamily 0 group B member 1; nuclear receptor subfamily 0, group B, member 1 NR0B1 AHC, AHCH, AHX, DAX-1, DAX1, DSS, GTD, HHG, NROB1, SRXY2 nuclear receptor subfamily 0 group B member 1 nuclear receptor subfamily 0 group B member 1|DSS-AHC critical region on the X chromosome protein 1|nuclear hormone receptor|nuclear receptor DAX-1|truncated nuclear receptor subfamily 0 group B member 1

For more info on NR0B1, check out the NR0B1 Infographic

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In this infographic, you will see the following information for NR0B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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