Product Info Summary
SKU: | A02069-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-DAZL Picoband™ Antibody
SKU/Catalog Number
A02069-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DAZL Picoband™ Antibody catalog # A02069-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DAZL Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02069-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human DAZL recombinant protein (Position: E34-R281).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02069-2 is reactive to DAZL in Human, Mouse, Rat
Applications
A02069-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
38 kDa
Calculated molecular weight
33.178kDa
Background of DAZL
Deleted in azoospermia-like is a protein that in humans is encoded by the DAZL gene. It is mapped to 3p24.3. The DAZ (Deleted in AZoospermia) gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3μg/1x106 cells, Human, Mouse
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of DAZL using anti-DAZL antibody (A02069-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: rat testicular tissue lysates,
Lane 3: mouse testicular tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAZL antigen affinity purified polyclonal antibody (Catalog # A02069-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAZL at approximately 38KD. The expected band size for DAZL is at 33KD.
Click image to see more details
Figure 2. IHC analysis of DAZL using anti-DAZL antibody (A02069-2).
DAZL was detected in paraffin-embedded section of human testies cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DAZL Antibody (A02069-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of DAZL using anti-DAZL antibody (A02069-2).
DAZL was detected in paraffin-embedded section of mouse testies cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DAZL Antibody (A02069-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of DAZL using anti-DAZL antibody (A02069-2).
DAZL was detected in paraffin-embedded section of rat testies cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DAZL Antibody (A02069-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. Flow Cytometry analysis of HEPA1-6 cells using anti-DAZL antibody (A02069-2).
Overlay histogram showing HEPA1-6 cells stained with A02069-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DAZL Antibody (A02069-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 6. Flow Cytometry analysis of HL-60 cells using anti-DAZL antibody (A02069-2).
Overlay histogram showing HL-60 cells stained with A02069-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DAZL Antibody (A02069-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For DAZL (Source: Uniprot.org, NCBI)
Gene Name
DAZL
Full Name
Deleted in azoospermia-like
Weight
33.178kDa
Superfamily
RRM DAZ family
Alternative Names
DAZHSPGY-like-autosomal; DAZL1DAZ-like autosomal; DAZLA; Deleted in azoospermia-like 1; deleted in azoospermia-like; germline specific RNA binding protein; MGC26406; spermatogenesis gene on the Y-like autosomal; SPGYLADAZ homolog DAZL DAZH1, DAZLA, SPGYLA, DAZL deleted in azoospermia like deleted in azoospermia-like|DAZ homolog|DAZ-like autosomal|SPGY-like-autosomal|deleted in azoospermia-like 1|germline specific RNA binding protein|spermatogenesis gene on the Y-like autosomal|testis secretory sperm-binding protein Li 204a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DAZL, check out the DAZL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DAZL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DAZL Picoband™ Antibody (A02069-2)
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