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Product Info Summary
| SKU: | A09406 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-DC-LAMP LAMP3 Antibody
SKU/Catalog Number
A09406
Size
100μl
Form
Liquid
Description
Boster Bio Anti-DC-LAMP LAMP3 Antibody catalog # A09406. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DC-LAMP LAMP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09406)
Host
Rabbit
Contents
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide from human protein at AA range: 261-310
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A09406 is reactive to LAMP3 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
44.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09406 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:500-200
ELISA 1:10000-20000
IF 1:50-200
Validation Images & Assay Conditions
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Western blot analysis of mouse-lung mouse-heart 293T Hela 3T3 lysate, antibody was diluted at 500. Secondary antibody was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:200
Specific Publications For Anti-DC-LAMP LAMP3 Antibody (A09406)
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Customer Q&As
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4 Customer Q&As for Anti-DC-LAMP LAMP3 Antibody
Question
Does anti-DC-LAMP antibody A09406 work for IHC with visceral pleura?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
According to the expression profile of visceral pleura, LAMP3 is highly expressed in visceral pleura. So, it is likely that anti-DC-LAMP antibody A09406 will work for IHC with visceral pleura.
Boster Scientific Support
Answered: 2019-11-01
Question
Would A09406 anti-DC-LAMP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
As indicated on the product datasheet, A09406 anti-DC-LAMP antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-27
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for visceral pleura using anti-DC-LAMP antibody A09406. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-11-15
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-15
Question
Does anti-DC-LAMP antibody A09406 work on goat IHC with visceral pleura?
Verified Customer
Verified customer
Asked: 2017-08-15
Answer
Our lab technicians have not validated anti-DC-LAMP antibody A09406 on goat. You can run a BLAST between goat and the immunogen sequence of anti-DC-LAMP antibody A09406 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat visceral pleura in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-15
