Anti-DGAT1 Antibody Picoband™

Product Name

Anti-DGAT1 Antibody Picoband™

View all DGAT1 Antibodies

SKU/Catalog Number

PB9801

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-DGAT1 Antibody Picoband™ catalog # PB9801. Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DGAT1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9801)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human DGAT1, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9801 is reactive to DGAT1 in Human, Rat

Applications

PB9801 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

60 kDa

Calculated molecular weight

55.278kDa

Background of DGAT1

Acyl-CoA: diacylglycerol acyltransferase (DGAT) is a microsomal enzyme that plays a central role in the metabolism of cellular diacylglycerol lipids and catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol (DAG) and fatty acyl CoA as substrates. DGAT had been considered necessary for adipose tissue formation and essential for survival. There are two isozymes of DGAT encoded by the genes DGAT1 and DGAT2. DGAT1 is a host factor for HCV infection that binds core protein, localizes it to DGAT1-generated lipid droplets, and recruits viral RNA replication complexes for viral assembly. DGAT2-generated lipid droplets formed normally in cells treated with the DGAT1 inhibitor, suggesting that DGAT1 inhibitors may be useful as antiviral therapeutics.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of DGAT1 using anti-DGAT1 antibody (PB9801).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Kidney Tissue Lysate at 50ug,
Lane 2: HELA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DGAT1 antigen affinity purified polyclonal antibody (Catalog # PB9801) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DGAT1 at approximately 60 kDa. The expected band size for DGAT1 is at 60 kDa.

Gene/Protein Information For DGAT1 (Source: Uniprot.org, NCBI)

Alternative Names

ACAT-related gene product 1; AGRP1; ARAT; ARGP1; ARGP1acyl coenzyme A:cholesterol acyltransferase related gene 1; DGAT; DGAT1; DGATACAT related gene product 1; diacylglycerol O-acyltransferase 1; diacylglycerol O-acyltransferase homolog 1 (mouse); diacylglycerol O-acyltransferase homolog 1; DIAR7; Diglyceride acyltransferase; EC 2.3.1; EC 2.3.1.20 DGAT1 ARAT, ARGP1, DGAT, DIAR7 diacylglycerol O-acyltransferase 1 diacylglycerol O-acyltransferase 1|ACAT related gene product 1|acyl coenzyme A:cholesterol acyltransferase related gene 1|acyl-CoA retinol O-fatty-acyltransferase|acyl-CoA:diacylglycerol acyltransferase|diglyceride acyltransferase

For more info on DGAT1, check out the DGAT1 Infographic

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In this infographic, you will see the following information for DGAT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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