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Product Info Summary
| SKU: | M00813 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-DHFR Monoclonal Antibody
View all Dihydrofolate Reductase/DHFR Antibodies
SKU/Catalog Number
M00813
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-DHFR Monoclonal Antibody catalog # M00813. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DHFR Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00813)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACBE-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human DHFR Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00813 is reactive to DHFR in Human, Mouse, Rat
Applications
M00813 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
180 kDa
Calculated molecular weight
21.453kDa
Background of Dihydrofolate Reductase/DHFR
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of DHFR expression in (1) HeLa cell lysate; (2) Mouse spleen lysate.
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All lanes use the Antibody at 1:8K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:8K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:8K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For DHFR (Source: Uniprot.org, NCBI)
Gene Name
DHFR
Full Name
Dihydrofolate reductase
Weight
21.453kDa
Superfamily
dihydrofolate reductase family
Alternative Names
DHFR; DHFRP1; Dihydrofolate Reductase; DYR; EC 1.5.1.3 DHFR DHFRP1, DYR dihydrofolate reductase dihydrofolate reductase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DHFR, check out the DHFR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DHFR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DHFR Monoclonal Antibody (M00813)
Hello CJ!
No publications found for M00813
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Recommended Resources
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
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16 Customer Q&As for Anti-DHFR Monoclonal Antibody
Question
I was wanting to use your anti-DHFR Monoclonal antibody for IF for rat buccal mucosa cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat buccal mucosa cell identification?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
As indicated on the product datasheet, M00813 anti-DHFR Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat buccal mucosa cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-28
Question
We are currently using anti-DHFR Monoclonal antibody M00813 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
The anti-DHFR Monoclonal antibody (M00813) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-24
Question
you antibody to test anti-DHFR Monoclonal antibody M00813 on rat buccal mucosa cell for research purposes, then I may be interested in using anti-DHFR Monoclonal antibody M00813 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
The products we sell, including anti-DHFR Monoclonal antibody M00813, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-05
Question
Our lab used your anti-DHFR Monoclonal antibody for IF on eye a few years ago. I am using mouse, and I plan to use the antibody for WB next. Our lab want to know about examining eye as well as buccal mucosa cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
I have checked the website and datasheets of our anti-DHFR Monoclonal antibody and it appears that M00813 has been validated on mouse in both IF and WB. Thus M00813 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-27
Question
Is a blocking peptide available for product anti-DHFR Monoclonal antibody (M00813)?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
We do provide the blocking peptide for product anti-DHFR Monoclonal antibody (M00813). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-11
Question
Will anti-DHFR Monoclonal antibody M00813 work for IF with buccal mucosa cell?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
According to the expression profile of buccal mucosa cell, DHFR is highly expressed in buccal mucosa cell. So, it is likely that anti-DHFR Monoclonal antibody M00813 will work for IF with buccal mucosa cell.
Boster Scientific Support
Answered: 2019-12-10
Question
Would M00813 anti-DHFR Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
It shows on the product datasheet, M00813 anti-DHFR Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-06
Question
See below the WB image, lot number and protocol we used for buccal mucosa cell using anti-DHFR Monoclonal antibody M00813. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-15
Question
My boss were satisfied with the WB result of your anti-DHFR Monoclonal antibody. However we have seen positive staining in buccal mucosa cell mitochondrion using this antibody. Is that expected? Could you tell me where is DHFR supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
From what I have seen in literature, buccal mucosa cell does express DHFR. Generally DHFR expresses in mitochondrion. Regarding which tissues have DHFR expression, here are a few articles citing expression in various tissues:
Eye, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-07-04
Question
Is there a BSA free version of anti-DHFR Monoclonal antibody M00813 available?
F. Li
Verified customer
Asked: 2019-03-29
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DHFR Monoclonal antibody M00813 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-03-29
Question
My question regarding product M00813, anti-DHFR Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-01-16
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00813 anti-DHFR Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-16
Question
Is this M00813 anti-DHFR Monoclonal antibody reactive to the isotypes of DHFR?
B. Krishna
Verified customer
Asked: 2018-07-09
Answer
The immunogen of M00813 anti-DHFR Monoclonal antibody is A synthesized peptide derived from human DHFR Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-09
Question
We have seen staining in human buccal mucosa cell. Are there any suggestions? Is anti-DHFR Monoclonal antibody supposed to stain buccal mucosa cell positively?
A. Mangal
Verified customer
Asked: 2018-02-19
Answer
Based on literature buccal mucosa cell does express DHFR. Based on Uniprot.org, DHFR is expressed in buccal mucosa cell, eye, among other tissues. Regarding which tissues have DHFR expression, here are a few articles citing expression in various tissues:
Eye, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-02-19
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for buccal mucosa cell using anti-DHFR Monoclonal antibody M00813. Let me know if you need anything else.
B. Anderson
Verified customer
Asked: 2017-10-02
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-02
Question
I was wanting to use using your anti-DHFR Monoclonal antibody for megaloblastic anemia due to dihydrofolate reductase studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-06-08
Answer
Thanks for your inquiry. This M00813 anti-DHFR Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-06-08
Question
I see that the anti-DHFR Monoclonal antibody M00813 works with IF, what is the protocol used to produce the result images on the product page?
M. Carter
Verified customer
Asked: 2015-03-20
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-03-20
