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Product Info Summary
| SKU: | P00475 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-DiGeorge Syndrome Critical Region 8 DGCR8 (Ser377) Antibody
SKU/Catalog Number
P00475
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-DiGeorge Syndrome Critical Region 8 DGCR8 (Ser377) Antibody (Catalog # P00475). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-DiGeorge Syndrome Critical Region 8 DGCR8 (Ser377) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00475)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser377 of human DGCR8, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00475 is reactive to DGCR8 in Human, Mouse
Applications
P00475 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
52 kDa
Calculated molecular weight
86.045kDa
Background of DGCR8
The Drosha-DGCR8 microprocessor complex is required for microRNA (miRNA) biogenesis. DGCR8 (DiGeorge Syndrome Critical Region 8) recognizes the RNA substrate, whereas Drosha functions as the endonuclease. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, interacts with the pri-miRNA and functions as the molecular anchor that measures the distance from the ds-RNA-ssRNA junction and directs Drosha cleavage 11bp away (Han, J., et al, 2006). The efficiency of Drosha cleavage increases in the presence of heme and promotes the formation of highly ordered DGCR8 structures upon binding to RNA (Faller, M., et al, 2010).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
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Western blot of mouse nuclei lysate showing specific immunolabeling of the ~120 kDa DGCR8 protein phosphorylated at in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
Protein Target Info & Infographic
Gene/Protein Information For DGCR8 (Source: Uniprot.org, NCBI)
Gene Name
DGCR8
Full Name
Microprocessor complex subunit DGCR8
Weight
86.045kDa
Alternative Names
C22orf12; DGCRK6chromosome 22 open reading frame 12; DiGeorge syndrome critical region 8; DiGeorge syndrome critical region gene 8; Gy1; microprocessor complex subunit DGCR8; pasha DGCR8 C22orf12, DGCRK6, Gy1, pasha DGCR8 microprocessor complex subunit microprocessor complex subunit DGCR8|DiGeorge syndrome critical region 8|DiGeorge syndrome critical region gene 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DGCR8, check out the DGCR8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DGCR8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-DiGeorge Syndrome Critical Region 8 DGCR8 (Ser377) Antibody (P00475)
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No publications found for P00475
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