Anti-Dnmt3b Rabbit Monoclonal Antibody Rabbit Monoclonal Antibody

DNMT3B antibody

Boster Bio Anti-Dnmt3b Rabbit Monoclonal Antibody Rabbit Monoclonal Antibody catalog # M00319. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00319
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, ICC, WB

Product Name

Anti-Dnmt3b Rabbit Monoclonal Antibody Rabbit Monoclonal Antibody

View all DNMT3B Antibodies

SKU/Catalog Number

M00319

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Dnmt3b Rabbit Monoclonal Antibody Rabbit Monoclonal Antibody catalog # M00319. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Dnmt3b Rabbit Monoclonal Antibody Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00319)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

FO-4

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Dnmt3b

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00319 is reactive to DNMT3B in Human, Mouse, Rat

Applications

M00319 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

84 kDa

Calculated molecular weight

95.751kDa

Background of DNMT3B

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For DNMT3B (Source: Uniprot.org, NCBI)

Gene Name

DNMT3B

Full Name

DNA (cytosine-5)-methyltransferase 3B

Weight

95.751kDa

Superfamily

class I-like SAM-binding methyltransferase superfamily

Alternative Names

DNA (cytosine-5-)-methyltransferase 3 beta; DNA (cytosine-5)-methyltransferase 3B; DNA methyltransferase HsaIIIB; DNA MTase HsaIIIB; DNMT3B; EC 2.1.1.37; ICF; ICF1; M.HsaIIIB DNMT3B ICF, ICF1, M.HsaIIIB DNA methyltransferase 3 beta DNA (cytosine-5)-methyltransferase 3B|DNA (cytosine-5-)-methyltransferase 3 beta|DNA MTase HsaIIIB|DNA cytosine-5--methyltransferase 3 beta|DNA methyltransferase HsaIIIB

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DNMT3B, check out the DNMT3B Infographic

DNMT3B infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DNMT3B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Dnmt3b Rabbit Monoclonal Antibody Rabbit Monoclonal Antibody

Question

We are currently using anti-Dnmt3b Rabbit Monoclonal antibody Rabbit Monoclonal antibody M00319 for rat tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-10

Answer

The anti-Dnmt3b Rabbit Monoclonal antibody Rabbit Monoclonal antibody (M00319) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-10

Question

I was wanting to use your anti-Dnmt3b Rabbit Monoclonal antibody Rabbit Monoclonal antibody for WB for human secondary oocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human secondary oocyte identification?

Verified Customer

Verified customer

Asked: 2019-06-10

Answer

As indicated on the product datasheet, M00319 anti-Dnmt3b Rabbit Monoclonal antibody Rabbit Monoclonal antibody has been validated for IF, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human secondary oocyte in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-10

Question

I am interested in to test anti-Dnmt3b Rabbit Monoclonal antibody Rabbit Monoclonal antibody M00319 on human secondary oocyte for research purposes, then I may be interested in using anti-Dnmt3b Rabbit Monoclonal antibody Rabbit Monoclonal antibody M00319 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-04-22

Answer

The products we sell, including anti-Dnmt3b Rabbit Monoclonal antibody Rabbit Monoclonal antibody M00319, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-04-22

Question

Does anti-Dnmt3b Rabbit Monoclonal antibody Rabbit Monoclonal antibody M00319 work on horse ICC with fetal testis small intestine?

Verified Customer

Verified customer

Asked: 2018-05-29

Answer

Our lab technicians have not tested anti-Dnmt3b Rabbit Monoclonal antibody Rabbit Monoclonal antibody M00319 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Dnmt3b Rabbit Monoclonal antibody Rabbit Monoclonal antibody M00319 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse fetal testis small intestine in ICC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-05-29

Order DetailsPrice
M00319

100μL

$370
M00319-30ul

30μL sample

$99
M00319-carrier-free

Carrier Free

$370

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M00319
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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