Anti-DRD4 Antibody Picoband™

Product Name

Anti-DRD4 Antibody Picoband™

View all Dopamine Receptor D4 Antibodies

SKU/Catalog Number

A00998-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-DRD4 Antibody Picoband™ catalog # A00998-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DRD4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00998-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse DRD4 recombinant protein (Position: A57-C387).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00998-2 is reactive to DRD4 in Mouse, Rat

Applications

A00998-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

48 kDa

Calculated molecular weight

43.901kDa

Background of Dopamine Receptor D4

D (4) dopamine receptor is a protein that in humans is encoded by the Drd4 gene. This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. And this subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities. Two transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Mouse, Rat
Flow Cytometry, 1-3μg/1x10⁶ cells, Mouse
Direct ELISA, 0.1-0.5μg/ml, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Drd4 using anti-Drd4 antibody (A00998-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Drd4 antigen affinity purified polyclonal antibody (Catalog # A00998-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Drd4 at approximately 48KD. The expected band size for Drd4 is at 48KD.

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Figure 2. Flow Cytometry analysis of C6 cells using anti-Drd4 antibody (A00998-2).
Overlay histogram showing C6 cells stained with A00998-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Drd4 Antibody (A00998-2, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For DRD4 (Source: Uniprot.org, NCBI)

Alternative Names

D(2C) dopamine receptor; D(4) dopamine receptor; D4DR; Dopamine D4 receptor; dopamine receptor D4; seven transmembrane helix receptor DRD4 D4DR dopamine receptor D4 D(4) dopamine receptor|D(2C) dopamine receptor|dopamine D4 receptor|seven transmembrane helix receptor

For more info on DRD4, check out the DRD4 Infographic

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In this infographic, you will see the following information for DRD4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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