Anti-E3 SUMO-protein ligase PIAS4 PIAS4 Antibody

Product Name

Anti-E3 SUMO-protein ligase PIAS4 PIAS4 Antibody

View all PIASy Antibodies

SKU/Catalog Number

A02994

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-E3 SUMO-protein ligase PIAS4 PIAS4 Antibody (Catalog # A02994). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

PIAS4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-E3 SUMO-protein ligase PIAS4 PIAS4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02994)

Host

Rabbit

Contents

PIAS4 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

PIAS4 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human PIAS4. The immunogen is located within amino acids 50 - 100 of PIAS4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Multiple isoforms of CITED2 are known to exist.

Reactive Species

A02994 is reactive to PIAS4 in Human, Mouse, Rat

Applications

A02994 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

56.504kDa

Background of PIASy

The PIAS (protein inhibitor of activated STAT) proteins play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS4. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS4 interacts with p53 and blocks its ability to induce Bax and p21 transcription. PIAS4 is also important in the control of the ubiquitin-dependent proteasomal degradation of the Ets-1 transcription factor. PIAS4 has been implicated in the DNA-damage response pathway and is thought to work in combination with PIAS1 for the productive association of 53BP1, BRCA1 and RNF168.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

PIAS4 antibody can be used for detection of PIAS4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of PIAS4 in HL60 cell lysate with PIAS4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.

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Immunohistochemistry of PIAS4 in rat brain tissue with PIAS4 antibody at 5 μg/mL.

Gene/Protein Information For PIAS4 (Source: Uniprot.org, NCBI)

Alternative Names

E3 SUMO-protein ligase PIAS4; FLJ12419; MGC35296; Piasg; PIAS-gamma; PIASY; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; protein inhibitor of activated STAT protein PIASy; protein inhibitor of activated STAT, 4; zinc finger, MIZ-type containing 6; ZMIZ6 PIAS4 PIAS-gamma, PIASY, Piasg, ZMIZ6 protein inhibitor of activated STAT 4 E3 SUMO-protein ligase PIAS4|RING-type E3 ubiquitin transferase PIAS4|protein inhibitor of activated STAT protein 4|protein inhibitor of activated STAT protein PIASy|protein inhibitor of activated STAT protein gamma|zinc finger, MIZ-type containing 6

For more info on PIAS4, check out the PIAS4 Infographic

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In this infographic, you will see the following information for PIAS4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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