Anti-Phospho-ECT2 T790 Antibody

Product Name

Anti-Phospho-ECT2 T790 Antibody

View all ECT2 Antibodies

SKU/Catalog Number

P01862

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Phospho-ECT2 T790 Antibody (Catalog # P01862). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Phospho-ECT2 T790 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01862)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ECT2 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P01862 is reactive to ECT2 in Human

Applications

P01862 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

103.505kDa

Background of ECT2

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. ECT2, also known as epithelial cell transforming sequence 2 oncogene, was originally isolated as a transforming gene from epithelial cells. ECT2 catalyzes guanine nucleotide exchange on the small GTPases, RhoA, Rac1, and Cdc42. ECT2 may be phosphorylated during G₂ and M phases, and phosphorylation may be required for its exchange activity. Unlike other known guanine nucleotide exchange factors for Rho GTPases, ECT2 exhibits nuclear localization in interphase, spreads throughout the cytoplasm in prometaphase, and is condensed in the midbody during cytokinesis. Expression of dominant-negative ECT2 or microinjection of affinity-purified anti-ECT2 antibody into interphase cells strongly inhibits cytokinesis. These results suggest that ECT2 is an important link between the cell cycle machinery and Rho signaling pathways involved in the regulation of cell division. Phosphorylation at T790 or S375 significantly affects the catalytic activity of ECT2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:40,000
IP: User optimized
WB: 1:1,000 - 1:4,000

Validation Images & Assay Conditions

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Western blot analysis of endogenous phosphorylated ECT2 expression in cell lysates from interphase (lane 1) and mitotic HeLa cells (lane 2). ECT2 was detected using rabbit anti-ECT2 pT790 Antigen Affinity purified polyclonal antibody (Catalog # P01862) at 1:1000. Despite specific staining of interphase cells, this reagent is believed to be phospho specific based on ELISA results using both phosphorylated and non-phosphorylated immunizing peptide. Personal Communication, Toru Miki, CCR-NCI, Bethesda, MD.

Gene/Protein Information For ECT2 (Source: Uniprot.org, NCBI)

Alternative Names

ARHGEF31; epithelial cell transforming sequence 2 oncogene; Epithelial cell-transforming sequence 2 oncogene; FLJ10461; MGC138291; protein ECT2 ECT2 ARHGEF31 epithelial cell transforming 2 protein ECT2|epithelial cell-transforming sequence 2 oncogene

For more info on ECT2, check out the ECT2 Infographic

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In this infographic, you will see the following information for ECT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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