Product Info Summary
SKU: | A00830T56-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-EEF2 (phospho-T56) Antibody
SKU/Catalog Number
A00830T56-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-EEF2 (phospho-T56) Antibody catalog # A00830T56-1. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EEF2 (phospho-T56) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00830T56-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 20-70 of Human eEF2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00830T56-1 is reactive to EEF2 in Human, Mouse, Rat
Applications
A00830T56-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
95.338kDa
Background of eEF-2
Two elongation factors (EF) EF-Tu and EF-2 participate in the elongation phase during protein biosynthesis on the ribosome and their functional cycles depend on GTP binding and its hydrolysis. EF-Tu (also designated mitochondrial precursor p43) and EF-2 are multidomain GTPases with essential functions in translation, and they both bind to the same site on the ribosome where their low intrinsic GTPase activities are strongly stimulated. EF-Tu plays a central role in the fast and accurate delivery of aminoacyl-tRNAs to the translating ribosome. In addition, EF-Tu protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anticodon on tRNA can be achieved. EF-2 supports the translocation of tRNAs and of mRNAs on the ribosome so that a new codon can be exposed for decoding.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry (IHC) analyzes of p-EEF2 (T56) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Click image to see more details
Western blot (WB) analysis of p-EEF2 (T56) pAb at 1:500 dilution
Lane1:K562 whole cell lysate(40ug)
Lane2:HEK293T whole cell lysate(10ug)
Lane3:The Liver tissue lysate of Rat(40ug)
Lane4:The Liver tissue lysate of Mouse(40ug)
Protein Target Info & Infographic
Gene/Protein Information For EEF2 (Source: Uniprot.org, NCBI)
Gene Name
EEF2
Full Name
Elongation factor 2
Weight
95.338kDa
Superfamily
TRAFAC class translation factor GTPase superfamily
Alternative Names
eEF2; eEF-2; EF-2; EF2EEF-2; elongation factor 2; eukaryotic translation elongation factor 2; polypeptidyl-tRNA translocase EEF2 EEF-2, EF-2, EF2, SCA26 eukaryotic translation elongation factor 2 elongation factor 2|epididymis secretory sperm binding protein|polypeptidyl-tRNA translocase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EEF2, check out the EEF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EEF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EEF2 (phospho-T56) Antibody (A00830T56-1)
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No publications found for A00830T56-1
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Customer Reviews
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