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Product Info Summary
| SKU: | M04387 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-eIF-2 alpha (pS51) EIF2S1 Rabbit Monoclonal Antibody, Clone#RM298
SKU/Catalog Number
M04387
Size
100uL
Form
Liquid
Description
Boster Bio Anti-eIF-2 alpha (pS51) EIF2S1 Rabbit Monoclonal Antibody, Clone#RM298 (Catalog # M04387). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-eIF-2 alpha (pS51) EIF2S1 Rabbit Monoclonal Antibody, Clone#RM298 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04387)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM298
Isotype
Rabbit IgG
Immunogen
A phospho-peptide corresponding to human Phospho- eIF-2a (Ser51)
Cross-reactivity
Reacts to human eIF-2a (eukaryotic translation initiation factor 2 subunit alpha) only when phosphorylated at Ser51. No cross-reactivity to eIF-2a without phosphorylation at Ser51. May also react to mouse or rat Phospho-eIF-2a (Ser51).
Reactive Species
M04387 is reactive to EIF2S1 in Human
Calculated molecular weight
36.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04387 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:100-1:200 dilution
WB: 1:100-1:200 dilution.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of HeLa cell lysate nontreated (-) or treated (+) with Calyculin A (CalA), using anti-phospho-eIF-2 (Ser51) rabbit monoclonal antibody (clone RM298) at a 1:200 dilution.
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IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using anti-phospho-eIF-2 (Ser51) rabbit monoclonal antibody (clone RM298) at a 1:200 dilution.
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IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human thyroid cancer tissue section using anti-phospho-eIF-2 (Ser51) rabbit monoclonal antibody (clone RM298) at a 1:200 dilution.
Specific Publications For Anti-eIF-2 alpha (pS51) EIF2S1 Rabbit Monoclonal Antibody, Clone#RM298 (M04387)
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