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Product Info Summary
| SKU: | A00690-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-EphB4 (E601) Antibody
SKU/Catalog Number
A00690-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-EphB4 (E601) Antibody catalog # A00690-2. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EphB4 (E601) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00690-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to Human EphB4.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00690-2 is reactive to EPHB4 in Human, Mouse, Rat
Calculated molecular weight
108.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00690-2 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
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Western blot (WB) analysis of EphB4 (E601) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(30ug) Lane2:HepG2 whole cell lysate(30ug) Lane3:3T3-L1 whole cell lysate(30ug) Lane4:PC12 whole cell lysate(30ug)
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Immunohistochemistry (IHC) analyzes of EphB4 (E601) pAb in paraffin-embedded human brain tissue.
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Immunofluorescence analysis of MCF-7 cells using EphB4 (E601) pAb at dilution of 1:200 (40x lens).
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Immunoprecipitation of MCF-7 cell lysates using EphB4 pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4) .Lane 1 is 30% input. The western blot was probed using EphB4 pAb.
Specific Publications For Anti-EphB4 (E601) Antibody (A00690-2)
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