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Product Info Summary
| SKU: | A04098 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Ephrin-B3 Antibody
SKU/Catalog Number
A04098
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Ephrin-B3 Antibody (Catalog # A04098). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ephrin-B3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04098)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Ephrin-B3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04098 is reactive to EFNB3 in Human, Mouse, Rat
Applications
A04098 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
35.835kDa
Background of Ephrin-B3
Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons By similarity.
Mitsutoshi Nakada, Cancer Res., Sep 2006; 66: 8492 - 8500.
Klas Kullander, Genes & Dev., Apr 2001; 15: 877.
M. Douglas Benson, PNAS, Jul 2005; 102: 10694 - 10699.
Stephanie R. Kadison, J. Neurosci., Aug 2006; 26: 8909 - 8914.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from SKOV3 cells, using Ephrin-B3 antibody A04098.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using Ephrin-B3 antibody A04098.
Protein Target Info & Infographic
Gene/Protein Information For EFNB3 (Source: Uniprot.org, NCBI)
Gene Name
EFNB3
Full Name
Ephrin-B3
Weight
35.835kDa
Superfamily
ephrin family
Alternative Names
EFL-6; Efnb3; Elk-L3; EPH-related receptor tyrosine kinase ligand 8; Ephrin B3; EphrinB3; Ephrin-B3; Epl8; EPLG8EPH-related receptor transmembrane ligand ELK-L3; LERK-8; LERK8EFL6; NLERK-2 EFNB3 EFL6, EPLG8, LERK8 ephrin B3 ephrin-B3|EPH-related receptor transmembrane ligand ELK-L3|eph-related receptor tyrosine kinase ligand 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EFNB3, check out the EFNB3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EFNB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ephrin-B3 Antibody (A04098)
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No publications found for A04098
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Recommended Resources
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- Boster's Pathways Library
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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13 Customer Q&As for Anti-Ephrin-B3 Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain cortex using anti-Ephrin-B3 antibody A04098. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-13
Question
Would anti-Ephrin-B3 antibody A04098 work for IHC with brain cortex?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
According to the expression profile of brain cortex, EFNB3 is highly expressed in brain cortex. So, it is likely that anti-Ephrin-B3 antibody A04098 will work for IHC with brain cortex.
Boster Scientific Support
Answered: 2020-01-24
Question
I was wanting to use your anti-Ephrin-B3 antibody for IHC for human brain cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain cortex identification?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
It shows on the product datasheet, A04098 anti-Ephrin-B3 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-22
Question
We are currently using anti-Ephrin-B3 antibody A04098 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-11
Answer
The anti-Ephrin-B3 antibody (A04098) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-11
Question
Would A04098 anti-Ephrin-B3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-05-02
Answer
It shows on the product datasheet, A04098 anti-Ephrin-B3 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-02
Question
Do you have a BSA free version of anti-Ephrin-B3 antibody A04098 available?
Verified Customer
Verified customer
Asked: 2018-01-16
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Ephrin-B3 antibody A04098 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-16
Question
I have attached the WB image, lot number and protocol we used for brain cortex using anti-Ephrin-B3 antibody A04098. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-11-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-21
Question
Will anti-Ephrin-B3 antibody A04098 work on canine IHC with testis?
M. Jackson
Verified customer
Asked: 2017-01-27
Answer
Our lab technicians have not validated anti-Ephrin-B3 antibody A04098 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Ephrin-B3 antibody A04098 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine testis in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-01-27
Question
I see that the anti-Ephrin-B3 antibody A04098 works with IHC, what is the protocol used to produce the result images on the product page?
S. Mangal
Verified customer
Asked: 2014-09-19
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-09-19
Question
Is a blocking peptide available for product anti-Ephrin-B3 antibody (A04098)?
B. Krishna
Verified customer
Asked: 2014-06-16
Answer
We do provide the blocking peptide for product anti-Ephrin-B3 antibody (A04098). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-06-16
Question
I am interested in to test anti-Ephrin-B3 antibody A04098 on human brain cortex for research purposes, then I may be interested in using anti-Ephrin-B3 antibody A04098 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Jones
Verified customer
Asked: 2013-10-10
Answer
The products we sell, including anti-Ephrin-B3 antibody A04098, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-10-10
Question
Can you help my question with product A04098, anti-Ephrin-B3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Evans
Verified customer
Asked: 2013-05-27
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04098 anti-Ephrin-B3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-05-27
Question
Is this A04098 anti-Ephrin-B3 antibody reactive to the isotypes of EFNB3?
E. Parker
Verified customer
Asked: 2013-03-05
Answer
The immunogen of A04098 anti-Ephrin-B3 antibody is Synthesized peptide derived from human Ephrin-B3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-03-05
