Anti-EPHX2 Antibody Picoband™

Product Name

Anti-EPHX2 Antibody Picoband™

View all EPHX2 Antibodies

SKU/Catalog Number

A01999-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-EPHX2 Antibody Picoband™ catalog # A01999-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EPHX2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01999-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human EPHX2, different from the related mouse sequence by seven amino acids, and from the related rat sequence by eight amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01999-1 is reactive to EPHX2 in Human, Mouse, Rat

Applications

A01999-1 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

63 kDa

Calculated molecular weight

62.616kDa

Background of EPHX2

Soluble epoxide hydrolase (sEH) is a bifunctional enzyme that in humans is encoded by the EPHX2 gene. It is mapped to 8p21.2-p21.1. This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of EPHX2 using anti-EPHX2 antibody (A01999-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: rat liver tissue lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: mouse liver tissue lysates,
Lane 5: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPHX2 antigen affinity purified polyclonal antibody (Catalog # A01999-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPHX2 at approximately 63 kDa. The expected band size for EPHX2 is at 63 kDa.

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Figure 2. Flow Cytometry analysis of PC-3 cells using anti-EPHX2 antibody (A01999-1).
Overlay histogram showing PC-3 cells stained with A01999-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EPHX2 Antibody (A01999-1,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For EPHX2 (Source: Uniprot.org, NCBI)

Alternative Names

Bifunctional Epoxide Hydrolase 2; CEH; Cytosolic epoxide hydrolase; EC 3.3.2.10; EPHX2; Epoxide Hydratase 2; Epoxide hydratase; Epoxide Hydrolase 2; epoxide hydrolase 2, cytoplasmic; Epoxide Hydrolase 2, Cytosolic; epoxide hydrolase, soluble; SEH; Soluble epoxide hydrolase EPHX2 ABHD20, CEH, SEH epoxide hydrolase 2 bifunctional epoxide hydrolase 2|epoxide hydratase|epoxide hydrolase 2, cytoplasmic|epoxide hydrolase 2, cytosolic|epoxide hydrolase, soluble

For more info on EPHX2, check out the EPHX2 Infographic

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In this infographic, you will see the following information for EPHX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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