Anti-ER-beta1 (Estrogen Receptor beta-1) ESR2 Monoclonal Antibody

Product Name

Anti-ER-beta1 (Estrogen Receptor beta-1) ESR2 Monoclonal Antibody

View all ER beta/NR3A2 Antibodies

SKU/Catalog Number

M00786

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-ER-beta1 (Estrogen Receptor beta-1) ESR2 Monoclonal Antibody (Catalog # M00786). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Monkey, Mouse, Rat, Pig, Horse, Sheep.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-ER-beta1 (Estrogen Receptor beta-1) ESR2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00786)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: ERb455

Isotype

IgG2a, kappa

Immunogen

C-terminus fragment of recombinant human estrogen receptor beta protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M00786 is reactive to ESR2 in Human, Monkey, Mouse, Pig, Rat, Sheep, Horse

Applications

M00786 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

59.216kDa

Background of ER beta/NR3A2

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Anti-ER-beta1 Mouse Monoclonal Antibody (ERb455).

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Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Anti-ER-beta1 Mouse Monoclonal Antibody (ERb455).

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Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with Anti-ER-beta1 Mouse Monoclonal Antibody (ERb455).

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Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Anti-ER-beta1 Mouse Monoclonal Antibody (ERb455).

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Flow Cytometry for human ER-beta on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER-beta1 Mouse Monoclonal Antibody (ERb455).

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Flow Cytometry of human ER beta on BT474 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled ER beta1 Mouse Monoclonal Antibody (ERb455).

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Western Blot Analysis of human MCF-7 cell lysate using Anti-ER-beta1 Mouse Monoclonal Antibody (ERb455).

Gene/Protein Information For ESR2 (Source: Uniprot.org, NCBI)

Alternative Names

ER beta; Erb; ER-beta; ESR2; ESR-BETA; ESTRB; estrogen receptor 2 (ER beta); estrogen receptor beta 4; estrogen receptor beta; NR3A2; NR3A2ESRB; Nuclear receptor subfamily 3 group A member 2 ESR2 ER-BETA, ESR-BETA, ESRB, ESTRB, Erb, NR3A2, ODG8 estrogen receptor 2 estrogen receptor beta|estrogen receptor beta 4|estrogen receptor beta splice variant, ERbeta2delta7|estrogen receptor beta splice variant, ERbeta4delta7|estrogen receptor beta splice variant, ERbeta6|estrogen receptor beta splice variant, ERbeta6delta7|estrogen receptor beta splice variant, ERbeta7|estrogen receptor beta splice variant, ERbeta7delta7|estrogen receptor beta splice variant, ERbetaEx. 4L|estrogen receptor beta splice variant, ERbetaEx. 6L|nuclear receptor subfamily 3 group A member 2|oestrogen receptor beta

For more info on ESR2, check out the ESR2 Infographic

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In this infographic, you will see the following information for ESR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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