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Product Info Summary
| SKU: | A02021-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ERAP1 Antibody
SKU/Catalog Number
A02021-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ERAP1 Antibody (Catalog # A02021-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
ERAP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-ERAP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02021-1)
Host
Rabbit
Contents
ERAP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ERAP1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ERAP. The immunogen is located within amino acids 730 - 780 of ERAP1 .
Cross-reactivity
ERAP1 antibody is human and mouse reactive. At least two isoforms of ERAP1 are known to exist; this antibody will detect both isoforms. ERAP1 antibody is predicted to not cross-react with ERAP2.
Reactive Species
A02021-1 is reactive to ERAP1 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
107.2 kDa
Background of ERAP1
The endoplasmic reticulum (ER) aminopeptidase 1 (ERAP1), a member of the peptidase M1 family, plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides (1,2). It is also designated as adipocyte-derived leucine aminopeptidase (A-LAP), puromycin-insensitive leucine-specific aminopeptidase (PILS-AP) and aminopeptidase regulator of TNFR1 shedding (ARTS-1) (3). ERAP1 is localized to the lumen of the ER and induced by interferon. It may be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney (3,4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02021-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ERAP1 antibody can be used for detection of ERAP1 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ERAP1 in SK-N-SH cell lysate with ERAP1 antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of ERAP1 in mouse brain tissue with ERAP1 antibody at 5 μg/mL.
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Immunofluorescence of ERAP1 in mouse brain tissue with ERAP1 antibody at 20 μg/mL.
Specific Publications For Anti-ERAP1 Antibody (A02021-1)
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