Anti-ErbB2 (HER2) Monoclonal Antibody

Boster Bio Anti-ErbB2 (HER2) Monoclonal Antibody catalog # M00010-1. Tested in IP, IF, IHC, WB applications. This antibody reacts with Human. Cited in 3 publication(s).

Product Info Summary

SKU: M00010-1
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IP, IF, IHC, WB

Product Name

Anti-ErbB2 (HER2) Monoclonal Antibody

See all ErbB2/Her2 products

SKU/Catalog Number

M00010-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-ErbB2 (HER2) Monoclonal Antibody catalog # M00010-1. Tested in IP, IF, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ErbB2 (HER2) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00010-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

BCI-5

Immunogen

A synthesized peptide derived from human ErbB2 (HER2).

Reactive Species

M00010-1 is reactive to ERBB2 in Human

Applications

M00010-1 is guaranteed for IP, IF, IHC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500~1:2000
IHC-P 1:50~1:200
ICC/IF 1:50~1:200
IP 1:50
FCM 1:50

Validation Images & Assay Conditions

Gene/Protein Information For ERBB2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P04626

Gene ID

2064

Gene Name

ERBB2

Full Name

Receptor tyrosine-protein kinase erbB-2

Weight

137.91kDa

Superfamily

protein kinase superfamily

Alternative Names

CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomade ERBB2 CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1 erb-b2 receptor tyrosine kinase 2 receptor tyrosine-protein kinase erbB-2|c-erb B2/neu protein|herstatin|human epidermal growth factor receptor 2|metastatic lymph node gene 19 protein|neuro/glioblastoma derived oncogene homolog|neuroblastoma/glioblastoma derived oncogene homolog|proto-oncogene Neu|proto-oncogene c-ErbB-2|tyrosine kinase-type cell surface receptor HER2|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2|v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2|v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ERBB2, check out the ERBB2 Infographic

ERBB2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ERBB2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00010-1 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Lapatinib-incorporated lipoprotein-like nanoparticles: preparation and a proposed breast cancer-targeting mechanism

Tong ZJ, Shi NY, Zhang ZJ, Yuan XD, Hong XM. Biosci Rep. 2017 Aug 2;37(4). pii: BSR20170121. doi: 10.1042/BSR20170121. Print 2017 Aug 31. Expression and prognostic value of HER-2/neu in primary breast cancer with sentinel lymph node metastasis

Tao L, Suhua C, Juanjuan C, Zongzhi Y, Juan X, Dandan Z. Virol J. 2011 Mar 11;8:114. Doi: 10.1186/1743-422X-8-114. In Vitro Study On Human Cytomegalovirus Affecting Early Pregnancy Villous Evt'S Invasion Function.

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16 Customer Q&As for Anti-ErbB2 (HER2) Monoclonal Antibody

Question

I see that the anti-ErbB2 (HER2) Monoclonal antibody M00010-1 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-02-26

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-02-26

Question

Will anti-ErbB2 (HER2) Monoclonal antibody M00010-1 work for IF with liver?

Verified Customer

Verified customer

Asked: 2020-01-07

Answer

According to the expression profile of liver, ERBB2 is highly expressed in liver. So, it is likely that anti-ErbB2 (HER2) Monoclonal antibody M00010-1 will work for IF with liver.

Boster Scientific Support

Answered: 2020-01-07

Question

I have a question about product M00010-1, anti-ErbB2 (HER2) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-31

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00010-1 anti-ErbB2 (HER2) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-31

Question

Do you have a BSA free version of anti-ErbB2 (HER2) Monoclonal antibody M00010-1 available?

Verified Customer

Verified customer

Asked: 2019-12-24

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ErbB2 (HER2) Monoclonal antibody M00010-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-24

Question

We have seen staining in human liver. Are there any suggestions? Is anti-ErbB2 (HER2) Monoclonal antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2019-12-16

Answer

From what I have seen in literature liver does express ERBB2. From what I have seen in Uniprot.org, ERBB2 is expressed in esophagus mucosa, brain, fetal brain, mammary carcinoma, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have ERBB2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 24722188
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 2992089

Boster Scientific Support

Answered: 2019-12-16

Question

We need to test anti-ErbB2 (HER2) Monoclonal antibody M00010-1 on human liver for research purposes, then I may be interested in using anti-ErbB2 (HER2) Monoclonal antibody M00010-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-06-27

Answer

The products we sell, including anti-ErbB2 (HER2) Monoclonal antibody M00010-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-06-27

Question

I have attached the WB image, lot number and protocol we used for liver using anti-ErbB2 (HER2) Monoclonal antibody M00010-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-06-19

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-19

Question

I am interested in using your anti-ErbB2 (HER2) Monoclonal antibody for raf/map kinase cascade studies. Has this antibody been tested with western blotting on skbr cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

We appreciate your inquiry. This M00010-1 anti-ErbB2 (HER2) Monoclonal antibody is validated on skbr cells. It is guaranteed to work for IP, IF, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-05-24

Question

Is a blocking peptide available for product anti-ErbB2 (HER2) Monoclonal antibody (M00010-1)?

R. Moore

Verified customer

Asked: 2019-05-07

Answer

We do provide the blocking peptide for product anti-ErbB2 (HER2) Monoclonal antibody (M00010-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-05-07

Question

My boss were well pleased with the WB result of your anti-ErbB2 (HER2) Monoclonal antibody. However we have seen positive staining in esophagus mucosa isoform 3: cytoplasm. nucleus. using this antibody. Is that expected? Could you tell me where is ERBB2 supposed to be expressed?

V. Zhao

Verified customer

Asked: 2018-12-03

Answer

From what I have seen in literature, esophagus mucosa does express ERBB2. Generally ERBB2 expresses in isoform 1: cell membrane, isoform 2: cytoplasm. nucleus., isoform 3: cytoplasm. nucleus. Regarding which tissues have ERBB2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 24722188
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 2992089

Boster Scientific Support

Answered: 2018-12-03

Question

Does M00010-1 anti-ErbB2 (HER2) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

S. Parker

Verified customer

Asked: 2018-03-07

Answer

It shows on the product datasheet, M00010-1 anti-ErbB2 (HER2) Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-03-07

Question

I was wanting to use your anti-ErbB2 (HER2) Monoclonal antibody for IF for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?

Verified Customer

Verified customer

Asked: 2018-01-24

Answer

As indicated on the product datasheet, M00010-1 anti-ErbB2 (HER2) Monoclonal antibody has been tested for IP, IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-01-24

Question

We have tried in the past anti-ErbB2 (HER2) Monoclonal antibody for IHC on cervix carcinoma erythroleukemia a few months ago. I am using human, and We intend to use the antibody for IP next. I am interested in examining cervix carcinoma erythroleukemia as well as esophagus mucosa in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?

Verified Customer

Verified customer

Asked: 2017-12-06

Answer

I looked at the website and datasheets of our anti-ErbB2 (HER2) Monoclonal antibody and I see that M00010-1 has been tested on human in both IHC and IP. Thus M00010-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-12-06

Question

Is this M00010-1 anti-ErbB2 (HER2) Monoclonal antibody reactive to the isotypes of ERBB2?

B. Wu

Verified customer

Asked: 2017-11-03

Answer

The immunogen of M00010-1 anti-ErbB2 (HER2) Monoclonal antibody is A synthesized peptide derived from human ErbB2 (HER2). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-11-03

Question

We are currently using anti-ErbB2 (HER2) Monoclonal antibody M00010-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?

B. Yang

Verified customer

Asked: 2015-09-15

Answer

The anti-ErbB2 (HER2) Monoclonal antibody (M00010-1) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-09-15

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-ErbB2 (HER2) Monoclonal antibody M00010-1. Let me know if you need anything else.

P. Rodriguez

Verified customer

Asked: 2015-07-06

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-07-06

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M00010-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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