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- Table of Contents
ERBB2 antibody (M00010-1) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Immunofluorescent analysis of SKBR cells, using ErbB2 (HER2) Antibody.
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Anti-ErbB2 (HER2) Monoclonal Antibody
ERBB2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
ERBB2 antibody was positively detected by IHC in:
Rat stomach tissue, human breast carcinoma tissue, Human esophageal carcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ERBB2 antibody was positively detected by ICC/IF in:
SKBR cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
FC
Rat
Tested
Recommend
Human
Recommend
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-ErbB2 (HER2) Monoclonal Antibody
View all ERBB2 Antibodies
View all ERBB2 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BCI-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ErbB2 (HER2).
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
185 kDa
Calculated Molecular Weight
137.9 kDa
Cite This Product
Anti-ErbB2 (HER2) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00010-1)
Protocols
Immunohistochemistry (IHC) protocol for ERBB2 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ERBB2 antibody
Publications
Application:
Lapatinib-incorporated lipoprotein-like nanoparticles: preparation and a proposed breast cancer-targeting mechanism
PMID: 24902791
Tong ZJ, Shi NY, Zhang ZJ, Yuan XD, Hong XM. Biosci Rep. 2017 Aug 2;37(4). pii: BSR20170121. doi: 10.1042/BSR20170121. Print 2017 Aug 31. Expression and prognostic value of HER-2/neu in primary breast cancer with sentinel lymph node metastasis
PMID: 28667103
Tao L, Suhua C, Juanjuan C, Zongzhi Y, Juan X, Dandan Z. Virol J. 2011 Mar 11;8:114. Doi: 10.1186/1743-422X-8-114. In Vitro Study On Human Cytomegalovirus Affecting Early Pregnancy Villous Evt'S Invasion Function.
PMID: 21392403
Review
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Product Promise
We promise Anti-ErbB2 (HER2) Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00010-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026