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- Table of Contents
MAPK1 antibody (M00030) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Anti-ERK2 Monoclonal Antibody
MAPK1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
MAPK1 antibody was positively detected by IHC in:
Human melanoma tissue, Human renal cancer tissue, Rat lung tissue, Mouse ovary tissue, Rat skin tissue, human gastric adenocarcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:40
FC 1:40
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
FC
Human
Recommend
Tested
Recommend
Recommend
Recommend
Recommend
Rat
Recommend
Tested
Recommend
Recommend
Recommend
Recommend
Mouse
Recommend
Tested
Recommend
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-ERK2 Monoclonal Antibody
View all MAPK1 Antibodies
View all MAPK1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
II-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ERK2 Act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of ERK2 requires its phosphorylation by upstream kinases. ERK2 is located in the cytoplasm of resting cells and translocates into the nucleus upon extracellular stimuli by active transport of a dimer. ERK2 is essential for placental development and ERK2 in the trophoblast compartment may be indispensable for the vascularization of the labyrinth.
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
41 kDa
Calculated Molecular Weight
41.4 kDa
Cite This Product
Anti-ERK2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00030)
Protocols
Immunohistochemistry (IHC) protocol for MAPK1 antibody
Publications
Application:
Species:
Ameliorative effects of epigallocatechin-3-gallate nanoparticles on 2,4-dinitrochlorobenzene induced atopic dermatitis: A potential mechanism of inflammation-related necroptosis
Published: 2022-08-09
Journal: Frontiers in Nutrition
Application: IHC
Impact Factor: 6.59
Species: Mouse
PMID: 36017227
MicroRNA181c inhibits prostate cancer cell growth and invasion by targeting multiple ERK signaling pathway components
Published: 2018-01-16
Journal: PROSTATE
Application: WB
Impact Factor: 3.35
Species: Human
PMID: 29341215
Icariin and icaritin stimulate the proliferation of SKBr3 cells through the GPER1-mediated modulation of the EGFR-MAPK signaling pathway
Published: 2014-04-03
Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Application: WB
Impact Factor: 1.88
Species: Human
PMID: 24718680
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Product Promise
We promise Anti-ERK2 Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00030
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026