Anti-ERK2 Monoclonal Antibody

Product Name

Anti-ERK2 Monoclonal Antibody

View all MAPK1 Antibodies

SKU/Catalog Number

M00030
BM3951 is an alternative SKU for this antibody, used in previous lots.

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-ERK2 Monoclonal Antibody catalog # M00030. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ERK2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00030)

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

II-13

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human ERK2 Act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of ERK2 requires its phosphorylation by upstream kinases. ERK2 is located in the cytoplasm of resting cells and translocates into the nucleus upon extracellular stimuli by active transport of a dimer. ERK2 is essential for placental development and ERK2 in the trophoblast compartment may be indispensable for the vascularization of the labyrinth.

Reactive Species

M00030 is reactive to MAPK1 in Human, Mouse, Rat

Observed Molecular Weight

41 kDa

Calculated molecular weight

41.4 kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M00030 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:40
FC 1:40

Positive Control

IHC: Human melanoma tissue, Human renal cancer tissue, Rat lung tissue, Mouse ovary tissue, Rat skin tissue, human gastric adenocarcinoma tissue

Validation Images & Assay Conditions

See full target information MAPK1

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