Anti-ERp57 (GRP58) Monoclonal Antibody

ERp57/PDIA3 antibody

Boster Bio Anti-ERp57 (GRP58) Monoclonal Antibody catalog # M01464-1. Tested in IP, IF, IHC, WB applications. This antibody reacts with Mouse, Rat.

Product Info Summary

SKU: M01464-1
Size: 200ug
Reactive Species: Bovine, Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat
Host: Mouse
Application: IP, IF, IHC, ICC, WB

Product Name

Anti-ERp57 (GRP58) Monoclonal Antibody

View all ERp57/PDIA3 Antibodies

SKU/Catalog Number

M01464-1

Size

200ug

Form

liquid

Description

Boster Bio Anti-ERp57 (GRP58) Monoclonal Antibody catalog # M01464-1. Tested in IP, IF, IHC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ERp57 (GRP58) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01464-1)

Host

Mouse

Contents

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Clonality

Monoclonal

Clone Number

Map.ERp57

Isotype

IgG1

Immunogen

Human recombinant ERp57 (Grp58)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~57kDa.

Reactive Species

M01464-1 is reactive to PDIA3 in Bovine, Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat

Applications

M01464-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

56.782kDa

Background of ERp57/PDIA3

ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB (1:2000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Validation Images & Assay Conditions

Gene/Protein Information For PDIA3 (Source: Uniprot.org, NCBI)

Gene Name

PDIA3

Full Name

Protein disulfide-isomerase A3

Weight

56.782kDa

Superfamily

protein disulfide isomerase family

Alternative Names

Disulfide isomerase ER-60; EC 5.3.4.1; endoplasmic reticulum P58; Endoplasmic reticulum resident protein 57; Endoplasmic reticulum resident protein 60; ER protein 60; ER60; ERp57; ERp5758 kDa glucose-regulated protein; ERp60; ERp6058 kDa microsomal protein; ERp61; glucose regulated protein, 58kDa; GRP57; GRP58; GRP58ER protein 57; HsT17083; P58; PDIA3; phospholipase C-alpha; PI-PLC; protein disulfide isomerase family A, member 3; protein disulfide isomerase-associated 3; protein disulfide-isomerase A3 PDIA3 ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HEL-S-269, HEL-S-93n, HsT17083, P58, PI-PLC protein disulfide isomerase family A member 3 protein disulfide-isomerase A3|58 kDa glucose-regulated protein|58 kDa microsomal protein|ER protein 57|ER protein 60|disulfide isomerase ER-60|endoplasmic reticulum P58|endoplasmic reticulum resident protein 57|endoplasmic reticulum resident protein 60|epididymis secretory protein Li 269|epididymis secretory sperm binding protein Li 93n|glucose regulated protein, 58kDa|phospholipase C-alpha|protein disulfide isomerase-associated 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PDIA3, check out the PDIA3 Infographic

PDIA3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PDIA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M01464-1

200ug

$435

Free Secondary Antibody

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M01464-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$435.00

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