Anti-ESM1 Antibody Picoband™

Product Name

Anti-ESM1 Antibody Picoband™

View all Endocan/ESM-1 Antibodies

SKU/Catalog Number

A04169-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ESM1 Antibody Picoband™ catalog # A04169-1. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ESM1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04169-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human ESM1 recombinant protein (Position: W20-R184).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04169-1 is reactive to ESM1 in Human

Applications

A04169-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

25 kDa

Calculated molecular weight

20.095kDa

Background of Endocan/ESM-1

Endothelial cell-specific molecule 1 is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. Two transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of ESM1 using anti-ESM1 antibody (A04169-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human ESM1 protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESM1 antigen affinity purified polyclonal antibody (Catalog # A04169-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESM1 at approximately 25KD. The expected band size for ESM1 is at 20KD.

Gene/Protein Information For ESM1 (Source: Uniprot.org, NCBI)

Alternative Names

Endocan; endothelial cell-specific molecule 1; ESM1; ESM-1; IGFBP-rp6 ESM1 endocan endothelial cell specific molecule 1 endothelial cell-specific molecule 1|ESM-1

For more info on ESM1, check out the ESM1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ESM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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