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Product Info Summary
| SKU: | A00057 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Estrogen receptor ESR1 Antibody
SKU/Catalog Number
A00057
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Estrogen receptor ESR1 Antibody (Catalog # A00057). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ESR1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Estrogen receptor ESR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00057)
Host
Rabbit
Contents
ESR1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ESR1 antibody was raised against an 18 amino acid peptide near the center of human ESR1. The immunogen is located within amino acids 250 - 300 of ESR1.
Cross-reactivity
ESR1 antibody is human, mouse and rat reactive.
Reactive Species
A00057 is reactive to ESR1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
66.2 kDa
Background of ESR1
Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors (1). Estrogen receptors, including ESR1, also known as ER-alpha and ESR2 (ER-beta), contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. ESR1 is a widely expressed nuclear protein and serves as a strong activator of estrogen responsive genes (1,2). Phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1), plays a large role in regulating ER alpha activity (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00057 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ESR1 antibody can be used for detection of ESR1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ESR1 in rat brain tissue lysate with ESR1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of ESR1 in rat brain tissue with ESR1 antibody at 5 μg/mL.
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Immunofluorescence of ESR1 in rat brain tissue with ESR1 antibody at 20 μg/mL.
Specific Publications For Anti-Estrogen receptor ESR1 Antibody (A00057)
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