Anti-EVER2 TMC8 Antibody

Product Name

Anti-EVER2 TMC8 Antibody

View all TMC8 Antibodies

SKU/Catalog Number

A06892-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-EVER2 TMC8 Antibody (Catalog # A06892-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

EVER2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-EVER2 TMC8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06892-1)

Host

Rabbit

Contents

EVER2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

EVER2 antibody was raised against a 14 amino acid synthetic peptide from near the center of human EVER2. The immunogen is located within amino acids 300 - 350 of EVER2.

Cross-reactivity

At least three isoforms of EVER2 are known to exist; this antibody will only recognize the larger isoform. EVER2 has no cross-reactivity to EVER1.

Reactive Species

A06892-1 is reactive to TMC8 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

81.6 kDa

Background of TMC8

Epidermodysplasia verruciformis (EV) is an autosomal recessive genodermatosis associated with a high risk of skin cancers resulting from a high susceptibility to infection by specific human papillomaviruses. Mutations in two homologous genes EVER1 and EVER2 cause the majority of EV cases. These two proteins form a complex and interact with the zinc transporter ZnT-1 in the endoplasmic reticulum. Cells lacking EVER2 accumulated higher levels of zinc in the nucleolus and nucleus compare to those cells with and intact EVER2 gene, indicating that one role of EVER2 is to regulate the intracellular distribution of zinc.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06892-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

EVER2 antibody can be used for detection of EVER2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information TMC8

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