Anti-Flap endonuclease 1 FEN1 Antibody

Product Name

Anti-Flap endonuclease 1 FEN1 Antibody

View all FEN-1 Antibodies

SKU/Catalog Number

A01484

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Flap endonuclease 1 FEN1 Antibody (Catalog # A01484). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Flap endonuclease 1 FEN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01484)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human FEN1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01484 is reactive to FEN1 in Human, Mouse, Rat

Applications

A01484 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

42.593kDa

Background of FEN-1

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

Murray J.M., Mol. Cell. Biol. 14:4878-4888(1994).
Hiraoka L.R., Genomics 25:220-225(1995).
Taylor T.D., Nature 440:497-500(2006).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using FEN1 antibody A01484.

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Western blot analysis of extracts from Jurkat cells, 293 cells and HUVEC cells, using FEN1 antibody A01484.

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Immunofluorescence analysis of HeLa cells, using FEN1 antibody A01484.

Gene/Protein Information For FEN1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.1; FEN1; FEN-1; FEN-1hFEN-1; flap structure-specific endonuclease 1DNase IV; Maturation factor 1; maturation factor-1; MF1flap endonuclease 1; RAD2 FEN1 FEN-1, MF1, RAD2 flap structure-specific endonuclease 1 flap endonuclease 1|DNase IV|maturation factor-1

For more info on FEN1, check out the FEN1 Infographic

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In this infographic, you will see the following information for FEN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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