Product Info Summary
SKU: | M11954-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-FOX3/NeuN Rbfox3 Monoclonal Antibody
View all RBFOX3/NeuN Antibodies
SKU/Catalog Number
M11954-1
Size
100ul
Description
Boster Bio Anti-FOX3/NeuN Rbfox3 Monoclonal Antibody catalog # M11954-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FOX3/NeuN Rbfox3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M11954-1)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
N-terminal 99 amino acids of human FOX3 expressed in and purified from E. coli
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M11954-1 is reactive to Rbfox3 in Human, Mouse, Rat
Applications
M11954-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
40.61kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000
IF/ICC and IHC: 1:1,000-1:2,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of whole brain tissue lysates using mouse mAb to FOX3/NeuN M11954-1, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain, [4] adult mouse brain. Note the strong twin bands corresponding to the two alternate transcripts of FOX3/NeuN protein with apparent SDS-PAGE molecular weights of 46 and 48kDa. As with other FOX3/NeuN antibodies, an additional band at ~70kDa is revealed in some lysates.
Click image to see more details
Immunofluorescent analysis of rat brain stem costained with mouse mAb to FOX3/NeuN M11954-1 in green, and chicken pAb to microtubule associated protein 2 (MAP2) in red. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, the brain was post fixed for 24 hours, cut to 45M, and free-floating sections were stained with the above antibodies. The Fox3/NeuN antibody selectively stains nuclei and the proximal cytoplasm of neuronal cells while the MAP2 antibody labels dendrites and overlaps with Fox3/NeuN staining in the perikarya of neurons.
Protein Target Info & Infographic
Gene/Protein Information For Rbfox3 (Source: Uniprot.org, NCBI)
Gene Name
Rbfox3
Full Name
RNA binding protein fox-1 homolog 3
Weight
40.61kDa
Alternative Names
FLJ56884; FLJ58356; Fox-1 homolog C; FOX3; FOX-3; hexaribonucleotide binding protein 3; HRNBP3; NeuN flow cytometry; NeuN; neuronal nuclei; RBFOX3; RNA binding protein fox-1 homolog 3; RNA binding protein, fox-1 homolog (C. elegans) 3 Rbfox3|Fox-3, Hrn, Hrnbp3, Ne, NeuN, Neuna60|RNA binding protein, fox-1 homolog (C. elegans) 3|RNA binding protein fox-1 homolog 3|fox-1 homolog C|hexaribonucleotide binding protein 3|neuN antigen|neuronal nuclear antigen A60|neuronal nuclei antigen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Rbfox3, check out the Rbfox3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Rbfox3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FOX3/NeuN Rbfox3 Monoclonal Antibody (M11954-1)
Hello CJ!
M11954-1 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Lanfen Chen,Wei Chen,Mengbei Zhang et al.Comparison of therapeutic effects of melatonin by two different routes in focal cerebral ischemic rats.Journal of Neurorestoratology 2019,07(01):47-53.
Species: Rat
M11954-1 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:100
Manganas LN, Durá I, Osenberg S, Semerci F, Tosun M, Mishra R, Parkitny L, Encinas JM, Maletic-Savatic M. BASP1 labels neural stem cells in the neurogenic niches of mammalian brain. Sci Rep. 2021 Mar 10;11(1):5546. doi: 10.1038/s41598-021-85129-1. PMID: 33692421; PMCID: PMC7970918.
Species: Human,Mouse
M11954-1 usage in article: APP:IHC, SAMPLE:HEAD TISSUE, DILUTION:1:200
xiangrong chen,Yile Zeng,Fan Wang et al.ADAM17 Aggravates the Inflammatory Response by Modulating Microglia Polarization Through the TGF-β1/Smad Pathway Following Experimental Traumatic Brain Injury,04 February 2021,PREPRINT (Version 1) available at
Species: Rat,Mouse
M11954-1 usage in article: APP:IF, SAMPLE:BONE TISSUE, DILUTION:1:100
Sevoflurane post-conditioning attenuates traumatic brain injury-induced neuronal apoptosis by promoting autophagy via the PI3K/AKT signaling pathway
Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats
Chen X, Wu S, Chen C, Xie B, Fang Z, Hu W, Chen J, Fu H, He H. J Neuroinflammation. 2017 Jul 24;14(1):143. doi: 10.1186/s12974-017-0917-3. Omega-3 polyunsaturated fatty acid supplementation attenuates microglial-induced inflammation by inhibiting ...
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