Anti-Follistatin-related protein 3 FSTL3 Antibody

Product Name

Anti-Follistatin-related protein 3 FSTL3 Antibody

View all Follistatin-related Gene Protein/FLRG/Fstl3 Antibodies

SKU/Catalog Number

PA1915

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Follistatin-related protein 3 FSTL3 Antibody catalog # PA1915. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Follistatin-related protein 3 FSTL3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1915)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human FSTL3, different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1915 is reactive to FSTL3 in Human, Mouse, Rat

Applications

PA1915 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

22-24 kDa

Calculated molecular weight

27.663kDa

Background of Follistatin-related Gene Protein/FLRG/Fstl3

FSTL3 (Follistatin-Like 3) also known as FLRG or FOLLISTATIN-RELATED GENE, is a member of the follistatin-module protein family, which is composed of extracellular matrix-associated glycoproteins though to act in a paracrine manner to bind morphogens or growth/differentiation factors and regulate their activity during development. The FSTL3 gene extends over 7 kbp and contains 5 exons. By Southern blot analysis of somatic cell hybrids and FISH, Hayette et al. (1998) localized the FSTL3 gene to chromosome 19p13. Using recombinant mouse Fstl3, Tsuchida et al. (2000) found that Fstl3bound both activin and BMP2 and had a higher affinity for activin. Overexpression of Fstl3 inhibited BMP2-induced cell signaling in a reporter assay. In addition to the translocation in a case of B-cell chronic lymphocytic leukemia from which FSTL3 was isolated, Hayette et al. (1998) also observed rearrangement of the FSTL3 gene in a case of B-cell non-Hodgkin lymphoma and in a case of B-cell mantle zone lymphoma, suggesting that FSTL3 may be involved in the leukemogenesis process.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of FSTL3 using anti-FSTL3 antibody (PA1915).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U2OS whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human K562 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSTL3 antigen affinity purified polyclonal antibody (Catalog # PA1915) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FSTL3 at approximately 22-24KD. The expected band size for FSTL3 is at 22KD.

Click image to see more details

Figure 2. Western blot analysis of FSTL3 using anti-FSTL3 antibody (PA1915).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat testis tissue lysates,
Lane 3: rat NRK whole cell lysates,
Lane 4: mouse liver tissue lysates,
Lane 5: mouse testis tissue lysates,
Lane 6: mouse lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSTL3 antigen affinity purified polyclonal antibody (Catalog # PA1915) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FSTL3 at approximately 22-24KD. The expected band size for FSTL3 is at 22KD.

Gene/Protein Information For FSTL3 (Source: Uniprot.org, NCBI)

Alternative Names

FLRG; FLRGFSRP; follistatin-like 3 (secreted glycoprotein); Follistatin-like 3; Follistatin-like protein 3; Follistatin-related gene protein; follistatin-related protein 3; FSTL3 FSTL3 FLRG, FSRP follistatin like 3 follistatin-related protein 3|follistatin-like 3 (secreted glycoprotein)|follistatin-like protein 3|follistatin-related gene protein

For more info on FSTL3, check out the FSTL3 Infographic

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In this infographic, you will see the following information for FSTL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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