Product Info Summary
SKU: | M00219 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-FTO Monoclonal Antibody
SKU/Catalog Number
M00219
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-FTO Monoclonal Antibody catalog # M00219. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FTO Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00219)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEAA-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FTO Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00219 is reactive to Fto in Human
Applications
M00219 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
58 kDa
Calculated molecular weight
58.007kDa
Background of Fto
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of FTO using anti-FTO antibody (M00219).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: human Daudi whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human T-47D whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FTO antigen affinity purified monoclonal antibody (Catalog # M00219) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FTO at approximately 58 kDa. The expected band size for FTO is at 58 kDa.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Protein Target Info & Infographic
Gene/Protein Information For Fto (Source: Uniprot.org, NCBI)
Gene Name
Fto
Full Name
Alpha-ketoglutarate-dependent dioxygenase FTO
Weight
58.007kDa
Superfamily
fto family
Alternative Names
Alpha-ketoglutarate-dependent dioxygenase FTO; EC 1.14.11.-; fat mass and obesity associated; Fat mass and obesity-associated protein; FTO; KIAA1752protein fto; MGC5149 Fto|AW743446, mKIAA1752|fat mass and obesity associated|alpha-ketoglutarate-dependent dioxygenase FTO|U6 small nuclear RNA (2-O-methyladenosine-N(6)-)-demethylase FTO|U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO|fat mass and obesity-associated protein|m6A(m)-demethylase FTO|mRNA (2-O-methyladenosine-N(6)-)-demethylase FTO|mRNA N(6)-methyladenosine demethylase FTO|protein fatso|tRNA N1-methyl adenine demethylase FTO
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Fto, check out the Fto Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Fto: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FTO Monoclonal Antibody (M00219)
Hello CJ!
No publications found for M00219
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-FTO Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-FTO Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-FTO Monoclonal Antibody
Question
We are currently using anti-FTO Monoclonal antibody M00219 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
The anti-FTO Monoclonal antibody (M00219) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-14