Product Info Summary
SKU: | A01989 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IF, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Gab1(Phospho-Tyr627) Antibody
SKU/Catalog Number
A01989
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Gab1(Phospho-Tyr627) Antibody (Catalog # A01989). Tested in WB, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Gab1(Phospho-Tyr627) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01989)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Immunogen
Peptide sequence around phosphorylation site of tyrosine 627 (V-E-Y(p)-L-D) derived from Human Gab1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01989 is reactive to GAB1 in Human, Mouse
Applications
A01989 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
76.616kDa
Background of GAB1
The protein encoded by Gab1 is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene.
Anders Kallin, et al. (2004) J. Biol. Chem ; 279: 17897 - 17904.
Hideto Kameda, et al. (2001) Cell Growth Differ ; 12: 307.
Masaki Osawa, et al. (2002) J. Cell Biol ; 158: 773.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 110kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of GAB1 expression in MCF7 lysate (lane 1), GAB1 Protein (lane 2) and GST protein (lane 3). GAB1 at 130KD was detected using rabbit anti-GAB1 polyclonal antibody (Catalog # A01989) at 1μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Click image to see more details
Western blot analysis of extracts from 293 cells untreated or treated with EGF using Gab1(Phospho-Tyr627) antibody A01989.
Click image to see more details
Immunofluorescence staining of methanol-fixed Hela cells using Gab1(Phospho-Tyr627) antibody A01989.
Protein Target Info & Infographic
Gene/Protein Information For GAB1 (Source: Uniprot.org, NCBI)
Gene Name
GAB1
Full Name
GRB2-associated-binding protein 1
Weight
76.616kDa
Superfamily
GAB family
Alternative Names
GRB2-associated binder 1; GRB2-associated binding protein 1; GRB2-associated-binding protein 1; Growth factor receptor bound protein 2-associated protein 1 GAB1 DFNB26 GRB2 associated binding protein 1 GRB2-associated-binding protein 1|GRB2-associated binder 1|deafness, autosomal recessive 26|growth factor receptor bound protein 2-associated protein 1|symbol withdrawn, see GAB1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GAB1, check out the GAB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GAB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Gab1(Phospho-Tyr627) Antibody (A01989)
Hello CJ!
No publications found for A01989
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Gab1(Phospho-Tyr627) Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Gab1(Phospho-Tyr627) Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question