Anti-GAPDH Antibody (monoclonal, 5A12)

Product Name

Anti-GAPDH Antibody (monoclonal, 5A12)

View all GAPDH Antibodies

SKU/Catalog Number

M00227-6

Size

100μl/vial

Form

Liquid

Description

Boster Bio Anti-GAPDH Antibody (monoclonal, 5A12) catalog # M00227-6. Tested in ELISA, Dot, WB applications. This antibody reacts with all species.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GAPDH Antibody (monoclonal, 5A12) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00227-6)

Host

Mouse

Contents

1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Clonality

Monoclonal

Clone Number

5A12

Isotype

IgG1

Immunogen

Synthetic peptide CRLEKPAKYDDIKK coupled to KLH

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00227-6 is reactive to GAPDH in All species

Applications

M00227-6 is guaranteed for Dot blot, ELISA, WB Boster Guarantee

Observed Molecular Weight

47 kDa

Calculated molecular weight

36.053kDa

Background of GAPDH

The enzyme glyceraldehydes-3-phosphate dehydrogenase is a tetramer of identical chains that catalyzes the reversible oxidative phosphorylation of glyceraldehydes-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide. This is an important energy-yielding step in carbohydrate metabolism. GAPDH is found in almost all species with low rate of evolutionary changes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

1:500-1:5000

Validation Images & Assay Conditions

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Western blot analysis of different concentration of Anti-GAPDH (5A12) Monoclonal Antibody
Antigen: 293T cell lysate
Line No.
1: 1 ug/mL
2: 0.5 ug/mL
3: 0.1 ug/mL
Blocking: Incubate the membrane with 5% Non-Fat Milk, PBS, 4 ℃ overnight
Antibody Incubation: Diluted antibody in 5% Non-Fat Milk, TBS, 0.1% Tween-20, RT, 30mins

Gene/Protein Information For GAPDH (Source: Uniprot.org, NCBI)

Alternative Names

1D4 GAPDH; aging-associated gene 9 protein; Cytoplasm Marker; EC 1.2.1; EC 1.2.1.12; EC 2.6.99.-; G3PD; G3PD; G3PDH; GAPD; GAPDH; GAPDPeptidyl-cysteine S-nitrosylase GAPDH; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38; OCT1 coactivator in S phase; Peptidyl-cysteine S-nitrosylase GAPDH GAPDH G3PD, GAPD, HEL-S-162eP glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde-3-phosphate dehydrogenase|OCAS, p38 component|Oct1 coactivator in S phase, 38 Kd component|aging-associated gene 9 protein|epididymis secretory sperm binding protein Li 162eP|peptidyl-cysteine S-nitrosylase GAPDH

For more info on GAPDH, check out the GAPDH Infographic

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In this infographic, you will see the following information for GAPDH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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