Product Info Summary
SKU: | PA1722 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Glucose Transporter GLUT4/SLC2A4 Antibody
SKU/Catalog Number
PA1722
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Glucose Transporter GLUT4/SLC2A4 Antibody catalog # PA1722. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glucose Transporter GLUT4/SLC2A4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1722)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human GLUT4, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1722 is reactive to SLC2A4 in Human, Mouse, Rat
Applications
PA1722 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
55 kDa
Calculated molecular weight
54.787kDa
Background of Glut4
Facilitated glucose transport by mammalian cells is not a property of a single protein but an activity associated with a family of structurally related proteins. Glucose transporter 4 is a insulin-responsive glucose transporter. It belongs to solute carrier family 2, member 1. Insulin alters the subcellular localization of GLUT4 vesicles in human muscle, and that this effect is impaired equally in insulin-resistant subjects with and without diabetes. A similar pattern of defects cause insulin resistance in human adipocytes.Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Validation Images & Assay Conditions
Click image to see more details
Anti-GLUT4 antibody, PA1722, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
Click image to see more details
Anti-GLUT4 antibody, PA1722, Western blotting
All lanes: Anti GLUT4 (PA1722) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 3: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 4: Mouse Skeletal Muscle Tissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Predicted bind size: 55 KD
Observed bind size: 55 KD
Click image to see more details
Figure 3. IHC analysis of GLUT4 using anti-GLUT4 antibody (PA1722).
GLUT4 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLUT4 Antibody (PA1722) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For SLC2A4 (Source: Uniprot.org, NCBI)
Gene Name
SLC2A4
Full Name
Solute carrier family 2, facilitated glucose transporter member 4
Weight
54.787kDa
Superfamily
major facilitator superfamily
Alternative Names
Glut4; GLUT-4; GLUT4Glucose transporter type 4, insulin-responsive; insulin-responsive glucose transporter type 4; SLC2A4; solute carrier family 2 (facilitated glucose transporter), member 4; solute carrier family 2, facilitated glucose transporter member 4 SLC2A4 GLUT4 solute carrier family 2 member 4 solute carrier family 2, facilitated glucose transporter member 4|GLUT-4|glucose transporter type 4, insulin-responsive|insulin-responsive glucose transporter type 4|solute carrier family 2 (facilitated glucose transporter), member 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC2A4, check out the SLC2A4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC2A4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Glucose Transporter GLUT4/SLC2A4 Antibody (PA1722)
Hello CJ!
PA1722 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Mo Z,Li L,Yu H,Wu Y,Li H.Coumarins ameliorate diabetogenic action of dexamethasone via Akt activation and AMPK signaling in skeletal muscle.J Pharmacol Sci.2019 Mar;139(3):151-157.doi:10.1016/j.jphs.2019.01.001.Epub 2019 Jan 23.PMID:30733181.
Species: Human,Mouse
PA1722 usage in article: APP:WB, SAMPLE:CERVICAL TISSUE, DILUTION:NA
Coumarins ameliorate diabetogenic action of dexamethasone via Akt activation and AMPK signaling in skeletal muscle Zejun Mo, et al. J Pharmacol Sci. 2019 Mar;139(3):151-157. doi: 10.1016/j.jphs.2019.01.001. Epub 2019 Jan 23.
lncRNA Ftx promotes aerobic glycolysis and tumor progression through the PPAR? pathway in hepatocellular carcinoma
Acupuncture Alters Expression of Insulin Signaling Related Molecules and Improves Insulin Resistance in OLETF Rats
Zhou X, Wu W, Chen J, Wang X, Wang Y. Nutr Metab (Lond). 2015 Mar 8;12:10. Doi: 10.1186/S12986-015-0006-5. Ecollection 2015. Amp-Activated Protein Kinase Is Required For The Anti-Adipogenic Effects Of Alpha-Linolenic Acid.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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17 Customer Q&As for Anti-Glucose Transporter GLUT4/SLC2A4 Antibody
Question
We need using your anti-Glucose Transporter GLUT4/SLC2A4 antibody for non-insulin-dependent (niddm) studies. Has this antibody been tested with western blotting on cardiac muscle tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
I appreciate your inquiry. This PA1722 anti-Glucose Transporter GLUT4/SLC2A4 antibody is tested on rat skeletal muscle tissue, cardiac muscle tissue, tissue lysate, mouse skeletal muscle tissue, hela whole cell lysate. It is guaranteed to work for IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-11
Question
I was wanting to use your anti-Glucose Transporter GLUT4/SLC2A4 antibody for IHC-P for mouse muscle of leg on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse muscle of leg identification?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
It shows on the product datasheet, PA1722 anti-Glucose Transporter GLUT4/SLC2A4 antibody has been tested for IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse muscle of leg in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-18
Question
I see that the anti-Glucose Transporter GLUT4/SLC2A4 antibody PA1722 works with IHC-P, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-05
Question
Would PA1722 anti-Glucose Transporter GLUT4/SLC2A4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
It shows on the product datasheet, PA1722 anti-Glucose Transporter GLUT4/SLC2A4 antibody as been tested on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-04
Question
I have attached the WB image, lot number and protocol we used for muscle of leg using anti-Glucose Transporter GLUT4/SLC2A4 antibody PA1722. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-19
Question
Do you have a BSA free version of anti-Glucose Transporter GLUT4/SLC2A4 antibody PA1722 available?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Glucose Transporter GLUT4/SLC2A4 antibody PA1722 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-13
Question
Is this PA1722 anti-Glucose Transporter GLUT4/SLC2A4 antibody reactive to the isotypes of SLC2A4?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The immunogen of PA1722 anti-Glucose Transporter GLUT4/SLC2A4 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human GLUT4 (491-509aa EQEVKPSTELEYLGPDEND), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-29
Question
Can you help my question with product PA1722, anti-Glucose Transporter GLUT4/SLC2A4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-10-04
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1722 anti-Glucose Transporter GLUT4/SLC2A4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-04
Question
My boss were happy with the WB result of your anti-Glucose Transporter GLUT4/SLC2A4 antibody. However we have been able to see positive staining in cervix carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is SLC2A4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-09-11
Answer
From literature, cervix carcinoma does express SLC2A4. Generally SLC2A4 expresses in cell membrane. Regarding which tissues have SLC2A4 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Colon, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Boster Scientific Support
Answered: 2018-09-11
Question
Will anti-Glucose Transporter GLUT4/SLC2A4 antibody PA1722 work on horse WB with cervix carcinoma?
H. Baker
Verified customer
Asked: 2018-08-17
Answer
Our lab technicians have not tested anti-Glucose Transporter GLUT4/SLC2A4 antibody PA1722 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Glucose Transporter GLUT4/SLC2A4 antibody PA1722 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-17
Question
We have been able to see staining in human cervix carcinoma. What should we do? Is anti-Glucose Transporter GLUT4/SLC2A4 antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
Based on literature cervix carcinoma does express SLC2A4. Based on Uniprot.org, SLC2A4 is expressed in muscle of leg, colon, cervix carcinoma, leukemic t-cell, among other tissues. Regarding which tissues have SLC2A4 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Colon, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Boster Scientific Support
Answered: 2018-08-02
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for muscle of leg using anti-Glucose Transporter GLUT4/SLC2A4 antibody PA1722. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-02-21
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-21
Question
I am interested in to test anti-Glucose Transporter GLUT4/SLC2A4 antibody PA1722 on mouse muscle of leg for research purposes, then I may be interested in using anti-Glucose Transporter GLUT4/SLC2A4 antibody PA1722 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-31
Answer
The products we sell, including anti-Glucose Transporter GLUT4/SLC2A4 antibody PA1722, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-31
Question
Is a blocking peptide available for product anti-Glucose Transporter GLUT4/SLC2A4 antibody (PA1722)?
Z. Lewis
Verified customer
Asked: 2016-06-06
Answer
We do provide the blocking peptide for product anti-Glucose Transporter GLUT4/SLC2A4 antibody (PA1722). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-06-06
Question
We are currently using anti-Glucose Transporter GLUT4/SLC2A4 antibody PA1722 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
R. Krishna
Verified customer
Asked: 2014-11-24
Answer
The anti-Glucose Transporter GLUT4/SLC2A4 antibody (PA1722) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-11-24
Question
We bought anti-Glucose Transporter GLUT4/SLC2A4 antibody for WB on colon in a previous experiment. I am using rat, and We are going to use the antibody for IHC-P next. Our lab want to know about examining colon as well as muscle of leg in our next experiment. Could give a recommendation on which antibody would work the best for IHC-P?
K. Parker
Verified customer
Asked: 2014-11-06
Answer
I have checked the website and datasheets of our anti-Glucose Transporter GLUT4/SLC2A4 antibody and it appears that PA1722 has been tested on rat in both WB and IHC-P. Thus PA1722 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-11-06
Question
Will anti-Glucose Transporter GLUT4/SLC2A4 antibody PA1722 work for IHC-P with muscle of leg?
O. Krishna
Verified customer
Asked: 2014-01-03
Answer
According to the expression profile of muscle of leg, SLC2A4 is highly expressed in muscle of leg. So, it is likely that anti-Glucose Transporter GLUT4/SLC2A4 antibody PA1722 will work for IHC-P with muscle of leg.
Boster Scientific Support
Answered: 2014-01-03