Anti-Glucose Transporter GLUT4/SLC2A4 Antibody

Product Name

Anti-Glucose Transporter GLUT4/SLC2A4 Antibody

View all Glut4 Antibodies

SKU/Catalog Number

PA1722

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Glucose Transporter GLUT4/SLC2A4 Antibody catalog # PA1722. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Glucose Transporter GLUT4/SLC2A4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1722)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human GLUT4, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1722 is reactive to SLC2A4 in Human, Mouse, Rat

Applications

PA1722 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

54.787kDa

Background of Glut4

Facilitated glucose transport by mammalian cells is not a property of a single protein but an activity associated with a family of structurally related proteins. Glucose transporter 4 is a insulin-responsive glucose transporter. It belongs to solute carrier family 2, member 1. Insulin alters the subcellular localization of GLUT4 vesicles in human muscle, and that this effect is impaired equally in insulin-resistant subjects with and without diabetes. A similar pattern of defects cause insulin resistance in human adipocytes.Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat

Validation Images & Assay Conditions

Click image to see more details

Anti-GLUT4 antibody, PA1722, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue

Click image to see more details

Anti-GLUT4 antibody, PA1722, Western blotting
All lanes: Anti GLUT4 (PA1722) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 3: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 4: Mouse Skeletal Muscle Tissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Predicted bind size: 55 KD
Observed bind size: 55 KD

Click image to see more details

Figure 3. IHC analysis of GLUT4 using anti-GLUT4 antibody (PA1722).
GLUT4 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLUT4 Antibody (PA1722) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For SLC2A4 (Source: Uniprot.org, NCBI)

Alternative Names

Glut4; GLUT-4; GLUT4Glucose transporter type 4, insulin-responsive; insulin-responsive glucose transporter type 4; SLC2A4; solute carrier family 2 (facilitated glucose transporter), member 4; solute carrier family 2, facilitated glucose transporter member 4 SLC2A4 GLUT4 solute carrier family 2 member 4 solute carrier family 2, facilitated glucose transporter member 4|GLUT-4|glucose transporter type 4, insulin-responsive|insulin-responsive glucose transporter type 4|solute carrier family 2 (facilitated glucose transporter), member 4

For more info on SLC2A4, check out the SLC2A4 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SLC2A4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the SLC2A4 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis