Product Info Summary
| SKU: | A09415 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat, Cat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-GNL3L Antibody
SKU/Catalog Number
A09415
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GNL3L Antibody catalog # A09415. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat, Cat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GNL3L Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09415)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GNL3L. AA range:71-120
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A09415 is reactive to GNL3L in Human, Mouse, Rat, Cat
Observed Molecular Weight
39 kDa
Calculated molecular weight
65.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09415 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Western Blot analysis of HT29 cells using GNL3L Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
Click image to see more details
Western Blot analysis of customer's (cat sample) using GNL3L Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
Click image to see more details
Western blot analysis of lysates from 293, HeLa, and HT-29 cells, using GNL3L Antibody. The lane on the right is blocked with the synthesized peptide.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human brain. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4° overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-GNL3L Antibody (A09415)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-GNL3L Antibody
Question
Is a blocking peptide available for product anti-GNL3L antibody (A09415)?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
We do provide the blocking peptide for product anti-GNL3L antibody (A09415). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-03-03
Question
Is there a BSA free version of anti-GNL3L antibody A09415 available?
M. Lewis
Verified customer
Asked: 2020-01-27
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-GNL3L antibody A09415 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-27
Question
We are currently using anti-GNL3L antibody A09415 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-08
Answer
The anti-GNL3L antibody (A09415) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-08
Question
Here is the WB image, lot number and protocol we used for lung using anti-GNL3L antibody A09415. Please let me know if you require anything else.
O. Li
Verified customer
Asked: 2015-10-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-10-06

