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Product Info Summary
| SKU: | A02234 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-GUSB Antibody (Center)
View all beta-Glucuronidase/GUSB Antibodies
SKU/Catalog Number
A02234
Size
80 µl
Description
Boster Bio Anti-GUSB Antibody (Center) (Catalog # A02234). Tested in IHC-P, Flow Cytometry, WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-GUSB Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02234)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This GUSB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335-362 amino acids from the Central region of human GUSB.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02234 is reactive to GUSB in Human, Mouse
Reconstitution
Calculated molecular weight
74732 Da
Background of beta-Glucuronidase/GUSB
The GUSB gene encodes beta-glucuronidase (EC 3.2.1.31), a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans (Shipley et al., 1993 [PubMed 7680524]). It is a tetrameric glycoprotein composed of identical subunits (Oshima et al., 1987 [PubMed 3468507]). The GUSB gene is mutated in mucopolysaccharidosis type VII (MPS7; MIM 253220).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02234 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:4000
IHC-P: 1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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All lanes : Anti-GUSB Antibody (Center) at 1:4000 dilution
Lane 1: HT-29 whole cell lysate
Lane 2: WiDr whole cell lysate
Lane 3: K562 whole cell lysate
Lane 4: A2058 whole cell lysate
Lane 5: HL-60 whole cell lysate
Lane 6: Mouse liver tissue lysate
Lane 7: Mouse lung tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 75 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of A02234 on paraffin-embedded Human liver tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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Immunohistochemical analysis of A02234 on paraffin-embedded Human colon carcinoma tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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GUSB Antibody (Center) (Cat. #A02234) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For GUSB (Source: Uniprot.org, NCBI)
Gene Name
GUSB
Full Name
Beta-glucuronidase
Weight
74732 Da
Superfamily
glycosyl hydrolase 2 family
Alternative Names
Beta-glucuronidase, Beta-G1, GUSB GUSB BG, MPS7 glucuronidase beta beta-glucuronidase|beta-D-glucuronidase|beta-G1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GUSB, check out the GUSB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GUSB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GUSB Antibody (Center) (A02234)
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