Anti-HAVCR2 Antibody

Product Name

Anti-HAVCR2 Antibody

View all TIM-3 Antibodies

SKU/Catalog Number

M00657-1

Size

50 µl

Description

Boster Bio Anti-HAVCR2 Antibody (Catalog # M00657-1). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-HAVCR2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00657-1)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

1827CT151.74.68

Isotype

IgG2b,k

Immunogen

This HAVCR2 antibody is generated from a mouse immunized with a recombinant protein of human HAVCR2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00657-1 is reactive to HAVCR2 in Human

Applications

M00657-1 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

33.394kDa

Background of TIM-3

Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:4000
FC: 1:25

Validation Images & Assay Conditions

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All lanes : Anti-HAVCR2 Antibody at 1:4000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: Daudi whole cell lysate
Lane 3: Human kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 33 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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Overlay histogram showing U-2 OS cells stained with M00657-1(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M00657-1, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2b (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Gene/Protein Information For HAVCR2 (Source: Uniprot.org, NCBI)

Alternative Names

CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; KIM-3; SPTCL; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; TIM3; TIM-3; TIMD3; TIMD-3; TIMD3KIM-3 HAVCR2 CD366, HAVcr-2, KIM-3, SPTCL, TIM3, TIMD-3, TIMD3, Tim-3 hepatitis A virus cellular receptor 2 hepatitis A virus cellular receptor 2|T cell immunoglobulin mucin 3|T-cell immunoglobulin and mucin domain-containing protein 3|T-cell immunoglobulin mucin family member 3|T-cell immunoglobulin mucin receptor 3|T-cell membrane protein 3|kidney injury molecule-3

For more info on HAVCR2, check out the HAVCR2 Infographic

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In this infographic, you will see the following information for HAVCR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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