Product Info Summary
SKU: | A01073 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Tyrosine-protein kinase HCK Hck Antibody
SKU/Catalog Number
A01073
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Tyrosine-protein kinase HCK Hck Antibody catalog # A01073. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tyrosine-protein kinase HCK Hck Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01073)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
4A4
Isotype
IgG
Immunogen
Synthesized peptide derived from human Hck around the non-phosphorylation site of Y410.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01073 is reactive to HCK in Human, Mouse, Rat
Applications
A01073 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
59.6kDa
Background of HCK
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
Silverman E, et al. Mol Cell Biol. 2004 Dec; 24(24): 10573-10583.
Andrew D, et al. Biochem J. 2006 February 1; 393(Pt 3): 715
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of specific cells using Hck Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For HCK (Source: Uniprot.org, NCBI)
Gene Name
HCK
Full Name
Tyrosine-protein kinase HCK
Weight
59.6kDa
Superfamily
protein kinase superfamily
Alternative Names
Bmk; EC 2.7.10; EC 2.7.10.2; Hck; Hctk; hemopoietic cell kinaseJTK9; JTK9; p59-HCK/p60-HCK; tyrosine-protein kinase HCK HCK JTK9, p59Hck, p61Hck HCK proto-oncogene, Src family tyrosine kinase tyrosine-protein kinase HCK|hematopoietic cell kinase|hemopoietic cell kinase|p59-HCK/p60-HCK
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HCK, check out the HCK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HCK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tyrosine-protein kinase HCK Hck Antibody (A01073)
Hello CJ!
No publications found for A01073
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Tyrosine-protein kinase HCK Hck Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Tyrosine-protein kinase HCK Hck Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-Tyrosine-protein kinase HCK Hck Antibody
Question
Is a blocking peptide available for product anti-Hck antibody (A01073)?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
We do provide the blocking peptide for product anti-Hck antibody (A01073). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-16
Question
Here is the WB image, lot number and protocol we used for b-cell lymph using anti-Hck antibody A01073. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-18
Question
Do you have a BSA free version of anti-Hck antibody A01073 available?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Hck antibody A01073 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-12
Question
Will anti-Hck antibody A01073 work on primate WB with b-cell lymph?
W. Parker
Verified customer
Asked: 2019-03-12
Answer
Our lab technicians have not validated anti-Hck antibody A01073 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Hck antibody A01073 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate b-cell lymph in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-12
Question
We are interested in to test anti-Hck antibody A01073 on rat b-cell lymph for research purposes, then I may be interested in using anti-Hck antibody A01073 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-11-13
Answer
The products we sell, including anti-Hck antibody A01073, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-13
Question
My question regarding product A01073, anti-Hck antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Kulkarni
Verified customer
Asked: 2016-08-25
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01073 anti-Hck antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-08-25
Question
I was wanting to use your anti-Hck antibody for WB for rat b-cell lymph on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat b-cell lymph identification?
J. Krishna
Verified customer
Asked: 2013-06-05
Answer
It shows on the product datasheet, A01073 anti-Hck antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat b-cell lymph in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-06-05