Anti-HDAC-2 (C-terminus) Antibody

Product Name

Anti-HDAC-2 (C-terminus) Antibody

View all Histone Deacetylase 2/HDAC2 Antibodies

SKU/Catalog Number

A00325

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-HDAC-2 (C-terminus) Antibody (Catalog # A00325). Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Primate.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is six (6) months from date of opening.

Cite This Product

Anti-HDAC-2 (C-terminus) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00325)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol

Clonality

Polyclonal

Isotype

IgG

Immunogen

HDAC2 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus region of human HDAC2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00325 is reactive to HDAC2 in Human, Mouse, Rat, Primate

Applications

A00325 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

55.364kDa

Background of Histone Deacetylase 2/HDAC2

Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDAC2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human. Anti-HDAC2 antibodies are ideal for researchers interested in Breast Cancer, Cancer, Chromatin Research, Epigenetics, Histone Deacetylases, and Stem Cell Markers research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:60,000
IHC: 1:100-1:500
IF Microscopy: 1:100-1:500
WB: 1 µg/ml

Validation Images & Assay Conditions

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Western blot analysis of HDAC2 expression in mouse brain extract (lane 1) and mouse brain extract blocked with peptide (lane 2). HDAC2 at 59KD was detected using rabbit anti-HDAC-2 Antigen Affinity purified polyclonal antibody (Catalog # A00325) at 1 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Gene/Protein Information For HDAC2 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.5.1.98; HD2; HDAC2; Histone Deacetylase 2; RPD3; transcriptional regulator homolog RPD3; YAF1; YY1-associated factor 1 HDAC2 HD2, KDAC2, RPD3, YAF1 histone deacetylase 2 histone deacetylase 2|YY1-associated factor 1|transcriptional regulator homolog RPD3

For more info on HDAC2, check out the HDAC2 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HDAC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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