Anti-Phospho-HDAC2 (Ser394) Antibody

Product Name

Anti-Phospho-HDAC2 (Ser394) Antibody

View all Histone Deacetylase 2/HDAC2 Antibodies

SKU/Catalog Number

P00325

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-HDAC2 (Ser394) Antibody (Catalog # P00325). Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-HDAC2 (Ser394) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00325)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser394 of human HDAC2, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00325 is reactive to HDAC2 in Mouse, Rat

Applications

P00325 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

55.364kDa

Background of Histone Deacetylase 2/HDAC2

Histone Deacetylase 2 (HDAC2) is part of a family of histone deacetylases that are responsible for deacetylation of lysine residues in the histone core. HDAC2 is classified as a class I histone deacetylase and is ubiquitously expressed throughout the body (Kee et al, 2008). It has been shown that HDAC2 plays an important role in cardiac hypertrophy (Eom et al, 2011). Phosphorylation of Ser-394 is responsible for the hypertrophy-associated activation of HDAC2, whereas intrinsic basal activity is maintained by phosphorylation of Ser-422 and Ser-424 (EOM et al, 2011).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

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Western blot of mouse heart lysate showing specific immunolabeling of the ~55 kDa HDAC2 protein phosphorylated at Ser³⁹⁴ in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 min).

Gene/Protein Information For HDAC2 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.5.1.98; HD2; HDAC2; Histone Deacetylase 2; RPD3; transcriptional regulator homolog RPD3; YAF1; YY1-associated factor 1 HDAC2 HD2, KDAC2, RPD3, YAF1 histone deacetylase 2 histone deacetylase 2|YY1-associated factor 1|transcriptional regulator homolog RPD3

For more info on HDAC2, check out the HDAC2 Infographic

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In this infographic, you will see the following information for HDAC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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